2kir

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{{Seed}}
 
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[[Image:2kir.png|left|200px]]
 
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==Solution structure of a designer toxin, mokatoxin-1==
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The line below this paragraph, containing "STRUCTURE_2kir", creates the "Structure Box" on the page.
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<StructureSection load='2kir' size='340' side='right'caption='[[2kir]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2kir]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KIR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kir OCA], [https://pdbe.org/2kir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kir RCSB], [https://www.ebi.ac.uk/pdbsum/2kir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kir ProSAT]</span></td></tr>
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{{STRUCTURE_2kir| PDB=2kir | SCENE= }}
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/2kir_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kir ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Venomous animals immobilize prey using protein toxins that act on ion channels and other targets of biological importance. Broad use of toxins for biomedical research, diagnosis, and therapy has been limited by inadequate target discrimination, for example, among ion channel subtypes. Here, a synthetic toxin is produced by a new strategy to be specific for human Kv1.3 channels, critical regulators of immune T cells. A phage display library of 11,200 de novo proteins is designed using the alpha-KTx scaffold of 31 scorpion toxin sequences known or predicted to bind to potassium channels. Mokatoxin-1 (moka1) is isolated by affinity selection on purified target. Moka1 blocks Kv1.3 at nanomolar levels that do not inhibit Kv1.1, Kv1.2, or KCa1.1. As a result, moka1 suppresses CD3/28-induced cytokine secretion by T cells without cross-reactive gastrointestinal hyperactivity. The 3D structure of moka1 rationalizes its specificity and validates the engineering approach, revealing a unique interaction surface supported on an alpha-KTx scaffold. This scaffold-based/target-biased strategy overcomes many obstacles to production of selective toxins.
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===Solution structure of a designer toxin, mokatoxin-1===
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A designer ligand specific for Kv1.3 channels from a scorpion neurotoxin-based library.,Takacs Z, Toups M, Kollewe A, Johnson E, Cuello LG, Driessens G, Biancalana M, Koide A, Ponte CG, Perozo E, Gajewski TF, Suarez-Kurtz G, Koide S, Goldstein SA Proc Natl Acad Sci U S A. 2009 Dec 10. PMID:20007782<ref>PMID:20007782</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20007782}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2kir" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20007782 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20007782}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2KIR is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KIR OCA].
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==Reference==
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<ref group="xtra">PMID:20007782</ref><references group="xtra"/>
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[[Category: Synthetic construct]]
[[Category: Synthetic construct]]
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[[Category: Biancalana, M.]]
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[[Category: Biancalana M]]
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[[Category: Goldstein, S.]]
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[[Category: Goldstein S]]
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[[Category: Koide, A.]]
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[[Category: Koide A]]
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[[Category: Koide, S.]]
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[[Category: Koide S]]
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[[Category: Takacs, Z.]]
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[[Category: Takacs Z]]
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[[Category: Moka1]]
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[[Category: Mokatoxin]]
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[[Category: Phage display]]
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[[Category: Scorpion]]
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[[Category: Toxin]]
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[[Category: Venom]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 23 08:35:15 2010''
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Current revision

Solution structure of a designer toxin, mokatoxin-1

PDB ID 2kir

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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