2xgi

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'''Unreleased structure'''
 
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The entry 2xgi is ON HOLD until Paper Publication
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==Crystal structure of Barley Beta-Amylase complexed with 3,4- epoxybutyl alpha-D-glucopyranoside==
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<StructureSection load='2xgi' size='340' side='right'caption='[[2xgi]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2xgi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hordeum_vulgare Hordeum vulgare]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XGI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XGI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EBQ:2-[(2R)-OXIRAN-2-YL]ETHYL+ALPHA-D-GLUCOPYRANOSIDE'>EBQ</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=J5B:(3R)-3-hydroxybutyl+alpha-D-glucopyranoside'>J5B</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xgi OCA], [https://pdbe.org/2xgi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xgi RCSB], [https://www.ebi.ac.uk/pdbsum/2xgi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xgi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMYB_HORVU AMYB_HORVU]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in germinating barley grains. As a precursor to in vivo studies, here we report systematic analysis of the reversible and irreversible inhibition of the major beta-amylase of the grain endosperm (BMY1). The molecular basis of inhibitor action was defined through high resolution X-ray crystallography studies of unliganded barley beta-amylase, as well as its complexes with glycone site binder disaccharide iminosugar G1M, irreversible inhibitors alpha-epoxypropyl and alpha-epoxybutyl glucosides, which target the enzyme's catalytic residues, and the aglycone site binders acarbose and alpha-cyclodextrin.
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Authors: Rejzek, M., Stevenson, C.E.M., Southard, A.M., Stanley, D., Denyer, K., Smith, A.M., Naldrett, M.J., Lawson, D.M., Field, R.A.
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Chemical genetics and cereal starch metabolism: structural basis of the non-covalent and covalent inhibition of barley beta-amylase.,Rejzek M, Stevenson CE, Southard AM, Stanley D, Denyer K, Smith AM, Naldrett MJ, Lawson DM, Field RA Mol Biosyst. 2010 Nov 18. PMID:21085740<ref>PMID:21085740</ref>
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Description: Crystal structure of Barley Beta-Amylase complexed with 3,4-epoxybutyl alpha-D-glucopyranoside
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xgi" style="background-color:#fffaf0;"></div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 23 08:40:55 2010''
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==See Also==
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*[[Amylase 3D structures|Amylase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Hordeum vulgare]]
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[[Category: Large Structures]]
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[[Category: Denyer K]]
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[[Category: Field RA]]
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[[Category: Lawson DM]]
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[[Category: Naldrett MJ]]
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[[Category: Rejzek M]]
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[[Category: Smith AM]]
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[[Category: Southard AM]]
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[[Category: Stanley D]]
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[[Category: Stevenson CEM]]

Current revision

Crystal structure of Barley Beta-Amylase complexed with 3,4- epoxybutyl alpha-D-glucopyranoside

PDB ID 2xgi

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