3ngg
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3ngg is ON HOLD Authors: Banigan, J.R., Mandal, K., Sawaya, M.R., Thammavongsa, V., Hendrickx, A.P.A. , Schneewind, O. , Yeates,T.O., Kent, S.B.H. ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==X-ray Structure of Omwaprin== | |
| + | <StructureSection load='3ngg' size='340' side='right'caption='[[3ngg]], [[Resolution|resolution]] 1.33Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3ngg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oxyuranus_microlepidotus Oxyuranus microlepidotus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NGG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NGG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.33Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ngg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ngg OCA], [https://pdbe.org/3ngg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ngg RCSB], [https://www.ebi.ac.uk/pdbsum/3ngg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ngg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/WAPA_OXYMI WAPA_OXYMI] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ng/3ngg_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ngg ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The 50-residue snake venom protein L-omwaprin and its enantiomer D-omwaprin were prepared by total chemical synthesis. Radial diffusion assays were performed against Bacillus megaterium and Bacillus anthracis; both L-omwaprin and D-omwaprin showed anti-bacterial activity against B. megaterium. The native protein enantiomer, made of L- amino acids, failed to crystallize readily. However, when a racemic mixture containing equal amounts of L-omwaprin and D-omwaprin was used, diffraction quality crystals were obtained. The racemic protein sample crystallized in the centrosymmetric space group P2(1)/c and its structure was determined at atomic resolution (1.33 A) by a combination of Patterson and direct methods based on the strong scattering from the sulfur atoms in the eight cysteine residues per protein. Racemic crystallography once again proved to be a valuable method for obtaining crystals of recalcitrant proteins, and for determining high resolution X-ray structures by direct methods. | ||
| - | + | Determination of the X-ray structure of the snake venom protein omwaprin by total chemical synthesis and racemic protein crystallography.,Banigan JR, Mandal K, Sawaya MR, Thammavongsa V, Hendrickx AP, Schneewind O, Yeates TO, H Kent SB Protein Sci. 2010 Jul 28. PMID:20669184<ref>PMID:20669184</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3ngg" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Oxyuranus microlepidotus]] | ||
| + | [[Category: Banigan JR]] | ||
| + | [[Category: Hendrickx APA]] | ||
| + | [[Category: Kent SBH]] | ||
| + | [[Category: Mandal K]] | ||
| + | [[Category: Sawaya MR]] | ||
| + | [[Category: Schneewind O]] | ||
| + | [[Category: Thammavongsa V]] | ||
| + | [[Category: Yeates TO]] | ||
Current revision
X-ray Structure of Omwaprin
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