3mmr

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{{Seed}}
 
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[[Image:3mmr.jpg|left|200px]]
 
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==Structure of Plasmodium falciparum Arginase in complex with ABH==
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The line below this paragraph, containing "STRUCTURE_3mmr", creates the "Structure Box" on the page.
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<StructureSection load='3mmr' size='340' side='right'caption='[[3mmr]], [[Resolution|resolution]] 2.14&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3mmr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MMR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.14&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABH:2(S)-AMINO-6-BORONOHEXANOIC+ACID'>ABH</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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{{STRUCTURE_3mmr| PDB=3mmr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mmr OCA], [https://pdbe.org/3mmr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mmr RCSB], [https://www.ebi.ac.uk/pdbsum/3mmr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mmr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARGI_PLAF7 ARGI_PLAF7] Catalyzes the hydrolysis of L-arginine into urea and L-ornithine, which is a precursor for polyamine biosynthesis (PubMed:15843155, PubMed:19456858, PubMed:20527960, PubMed:21728378). May play a role in parasite intra-hepatic development during the host liver stage (By similarity).[UniProtKB:A0A509AF89]<ref>PMID:15843155</ref> <ref>PMID:19456858</ref> <ref>PMID:20527960</ref> <ref>PMID:21728378</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mm/3mmr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mmr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 2.15 A resolution crystal structure of arginase from Plasmodium falciparum, the parasite that causes cerebral malaria, is reported in complex with the boronic acid inhibitor 2(S)-amino-6-boronohexanoic acid (ABH) (K(d) = 11 muM). This is the first crystal structure of a parasitic arginase. Various protein constructs were explored to identify an optimally active enzyme form for inhibition and structural studies and to probe the structure and function of two polypeptide insertions unique to malarial arginase: a 74-residue low-complexity region contained in loop L2 and an 11-residue segment contained in loop L8. Structural studies indicate that the low-complexity region is largely disordered and is oriented away from the trimer interface; its deletion does not significantly compromise enzyme activity. The loop L8 insertion is located at the trimer interface and makes several intra- and intermolecular interactions important for enzyme function. In addition, we also demonstrate that arg- Plasmodium berghei sporozoites show significantly decreased liver infectivity in vivo. Therefore, inhibition of malarial arginase may serve as a possible candidate for antimalarial therapy against liver-stage infection, and ABH may serve as a lead for the development of inhibitors.
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===Structure of Plasmodium falciparum Arginase in complex with ABH===
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Crystal Structure of Arginase from Plasmodium falciparum and Implications for l-Arginine Depletion in Malarial Infection .,Dowling DP, Ilies M, Olszewski KL, Portugal S, Mota MM, Llinas M, Christianson DW Biochemistry. 2010 Jun 9. PMID:20527960<ref>PMID:20527960</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3mmr" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20527960}}, adds the Publication Abstract to the page
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*[[Arginase 3D structures|Arginase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20527960 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20527960}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3MMR is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum_3d7 Plasmodium falciparum 3d7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MMR OCA].
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[[Category: Plasmodium falciparum 3D7]]
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[[Category: Christianson DW]]
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==Reference==
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[[Category: Dowling DP]]
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<ref group="xtra">PMID:20527960</ref><references group="xtra"/>
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[[Category: Ilies M]]
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[[Category: Arginase]]
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[[Category: Llinas M]]
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[[Category: Plasmodium falciparum 3d7]]
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[[Category: Mota MM]]
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[[Category: Christianson, D W.]]
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[[Category: Olszewski KL]]
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[[Category: Dowling, D P.]]
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[[Category: Portugal S]]
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[[Category: Ilies, M.]]
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[[Category: Llinas, M.]]
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[[Category: Mota, M M.]]
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[[Category: Olszewski, K L.]]
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[[Category: Portugal, S.]]
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[[Category: Abh]]
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[[Category: Binuclear]]
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[[Category: Boronic acid]]
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[[Category: Hydrolase]]
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[[Category: L-arginine]]
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[[Category: Lcr]]
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[[Category: Malaria]]
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[[Category: Manganese]]
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[[Category: Metallohydrolase]]
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[[Category: Parasite]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 23 08:51:47 2010''
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Current revision

Structure of Plasmodium falciparum Arginase in complex with ABH

PDB ID 3mmr

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