3kxd

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{{Seed}}
 
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[[Image:3kxd.png|left|200px]]
 
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==Crystal structure of the mthk rck in complex with cadmium==
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The line below this paragraph, containing "STRUCTURE_3kxd", creates the "Structure Box" on the page.
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<StructureSection load='3kxd' size='340' side='right'caption='[[3kxd]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3kxd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._Delta_H Methanothermobacter thermautotrophicus str. Delta H]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KXD FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
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{{STRUCTURE_3kxd| PDB=3kxd | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kxd OCA], [https://pdbe.org/3kxd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kxd RCSB], [https://www.ebi.ac.uk/pdbsum/3kxd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kxd ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH] Calcium-gated potassium channel.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kx/3kxd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kxd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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RCK is a cytoplasmic regulatory domain of calcium-gated potassium channels. Binding of Ca(2+) by RCK leads to channel activation through a series of yet unknown conformational changes. Structures of the K(+) channel, MthK, and its cytoplasmic RCK domain revealed two binding sites for Ca(2+) per dimer. We determined the crystal structure of RCK in complex with Cd(2+) at 2.2A resolution. Cd(2+) activates MthK more efficiently, and binds at the same binding sites for Ca(2+) but with reduced coordination number. Two additional binding sites for Cd(2+) are found per dimer; one on the main Rossman-fold lobe, and the other on the small lobe of RCK. Using patch-clamp experiments, we demonstrate that Cd(2+) binding to these novel sites enhances activation by Cd(2+) and not by Ca(2+). The structure reveals a large negatively charged surface patch in the proximity of the Ca(2+)/Cd(2+) binding sites, charge neutralization of which appears to promote the channel open state.
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===Crystal structure of the mthk rck in complex with cadmium===
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Structure of the MthK RCK in complex with cadmium.,Dvir H, Valera E, Choe S J Struct Biol. 2010 Aug;171(2):231-7. Epub 2010 Apr 4. PMID:20371380<ref>PMID:20371380</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3kxd" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20371380}}, adds the Publication Abstract to the page
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20371380 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20371380}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3KXD is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._delta_h Methanothermobacter thermautotrophicus str. delta h]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXD OCA].
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[[Category: Methanothermobacter thermautotrophicus str. Delta H]]
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[[Category: Choe S]]
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==Reference==
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[[Category: Dvir H]]
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<ref group="xtra">PMID:20371380</ref><references group="xtra"/>
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[[Category: Methanothermobacter thermautotrophicus str. delta h]]
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[[Category: Choe, S.]]
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[[Category: Dvir, H.]]
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[[Category: Alternative initiation]]
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[[Category: Cadmium]]
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[[Category: Cell membrane]]
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[[Category: Ion transport]]
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[[Category: Ionic channel]]
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[[Category: Membrane]]
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[[Category: Metal-binding]]
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[[Category: Mthk]]
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[[Category: Potassium]]
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[[Category: Potassium channel]]
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[[Category: Potassium transport]]
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[[Category: Rck]]
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[[Category: Transmembrane]]
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[[Category: Transport]]
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[[Category: Transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 30 13:25:21 2010''
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Current revision

Crystal structure of the mthk rck in complex with cadmium

PDB ID 3kxd

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