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2xea

From Proteopedia

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{{Seed}}
 
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[[Image:2xea.jpg|left|200px]]
 
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==4.6 ANGSTROM CRYO-EM RECONSTRUCTION OF TOBACCO MOSAIC VIRUS FROM IMAGES RECORDED AT 300 KEV ON A 4KX4K CCD CAMERA==
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The line below this paragraph, containing "STRUCTURE_2xea", creates the "Structure Box" on the page.
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<SX load='2xea' size='340' side='right' viewer='molstar' caption='[[2xea]], [[Resolution|resolution]] 4.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xea]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tobacco_mosaic_virus Tobacco mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XEA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XEA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xea FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xea OCA], [https://pdbe.org/2xea PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xea RCSB], [https://www.ebi.ac.uk/pdbsum/2xea PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xea ProSAT]</span></td></tr>
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{{STRUCTURE_2xea| PDB=2xea | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q77LT8_9VIRU Q77LT8_9VIRU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xe/2xea_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xea ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a helical form with RNA (X-ray fibre diffraction) and as a second distinct helical form with RNA (cryo-EM). Here we present a structural analysis of TMV as a test object to assess the quality of cryo-EM images recorded at 300 keV on a CCD camera. The 4.6A TMV structure obtained is consistent with the previous cryo-EM structure and confirms that there is a second helical form of TMV. The structure here also shows that with a similar number of TMV segments an equivalent resolution can be achieved with a 4k CCD camera at 300 keV.
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===4.6 ANGSTROM CRYO-EM RECONSTRUCTION OF TOBACCO MOSAIC VIRUS FROM IMAGES RECORDED AT 300 KEV ON A 4KX4K CCD CAMERA===
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4.6A Cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300 keV on a 4k x 4k CCD camera.,Clare DK, Orlova EV J Struct Biol. 2010 Sep;171(3):303-8. Epub 2010 Jun 15. PMID:20558300<ref>PMID:20558300</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xea" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20558300}}, adds the Publication Abstract to the page
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*[[Sandbox TMV|Sandbox TMV]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20558300 is the PubMed ID number.
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*[[Tobacco Mosaic Virus|Tobacco Mosaic Virus]]
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== References ==
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{{ABSTRACT_PUBMED_20558300}}
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<references/>
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__TOC__
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==About this Structure==
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</SX>
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2XEA is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Tobacco_mosaic_virus Tobacco mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XEA OCA].
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:20558300</ref><references group="xtra"/>
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[[Category: Tobacco mosaic virus]]
[[Category: Tobacco mosaic virus]]
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[[Category: Clare, D K.]]
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[[Category: Clare DK]]
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[[Category: Orlova, E V.]]
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[[Category: Orlova EV]]
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[[Category: Single particle processing]]
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[[Category: Virus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 30 13:45:15 2010''
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Current revision

4.6 ANGSTROM CRYO-EM RECONSTRUCTION OF TOBACCO MOSAIC VIRUS FROM IMAGES RECORDED AT 300 KEV ON A 4KX4K CCD CAMERA

2xea, resolution 4.60Å

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