2cc2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (14:12, 13 December 2023) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2cc2.gif|left|200px]]<br /><applet load="2cc2" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2cc2, resolution 2.00&Aring;" />
 
-
'''X-RAY CRYSTAL STRUCTURE OF 5'-FLUORODEOXYADENOSINE SYNTHASE FROM STREPTOMYCES CATTLEYA COMPLEXED WITH 5'DEOXYADENOSINE'''<br />
 
-
==About this Structure==
+
==X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with 5'deoxyadenosine==
-
2CC2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya] with CL and 5AD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenosyl-fluoride_synthase Adenosyl-fluoride synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.63 2.5.1.63] Known structural/functional Site: <scene name='pdbsite=AC1:5ad Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CC2 OCA].
+
<StructureSection load='2cc2' size='340' side='right'caption='[[2cc2]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
[[Category: Adenosyl-fluoride synthase]]
+
== Structural highlights ==
-
[[Category: Single protein]]
+
<table><tr><td colspan='2'>[[2cc2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_cattleya Streptomyces cattleya]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CC2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CC2 FirstGlance]. <br>
-
[[Category: Streptomyces cattleya]]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
[[Category: Deng, H.]]
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-
[[Category: Hagan, D.O.]]
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cc2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cc2 OCA], [https://pdbe.org/2cc2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cc2 RCSB], [https://www.ebi.ac.uk/pdbsum/2cc2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cc2 ProSAT]</span></td></tr>
-
[[Category: Mcewan, A.R.]]
+
</table>
-
[[Category: Mcglinchey, R.P.]]
+
== Function ==
-
[[Category: Naismith, J.H.]]
+
[https://www.uniprot.org/uniprot/FLA_STRCT FLA_STRCT] Involved in the biosynthesis of fluorometabolites. Catalyzes the formation of a C-F bond by combining S-adenosyl-L-methionine (SAM) and fluoride to generate 5'-fluoro-5'-deoxyadenosine (5'-FDA) and L-methionine. It can also use 2'-deoxyadenosine in place of adenosine as substrate.<ref>PMID:12860396</ref> <ref>PMID:14765200</ref> <ref>PMID:16370017</ref> <ref>PMID:16604208</ref> <ref>PMID:16720268</ref> <ref>PMID:17985882</ref>
-
[[Category: Robinson, D.R.]]
+
== Evolutionary Conservation ==
-
[[Category: Spencer, J.]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: 5AD]]
+
Check<jmol>
-
[[Category: CL]]
+
<jmolCheckbox>
-
[[Category: 5'deoxyadenosine]]
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cc/2cc2_consurf.spt"</scriptWhenChecked>
-
[[Category: fla]]
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
[[Category: fluorinase]]
+
<text>to colour the structure by Evolutionary Conservation</text>
-
[[Category: transferase]]
+
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cc2 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:17:23 2007''
+
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.,Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA Org Biomol Chem. 2006 Apr 21;4(8):1458-60. Epub 2006 Mar 8. PMID:16604208<ref>PMID:16604208</ref>
 +
 
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2cc2" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Streptomyces cattleya]]
 +
[[Category: Deng H]]
 +
[[Category: McGlinchey RP]]
 +
[[Category: Mcewan AR]]
 +
[[Category: Naismith JH]]
 +
[[Category: O'Hagan D]]
 +
[[Category: Robinson DR]]
 +
[[Category: Spencer J]]

Current revision

X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with 5'deoxyadenosine

PDB ID 2cc2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2cc2"
Personal tools