3n26

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{{Seed}}
 
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[[Image:3n26.png|left|200px]]
 
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==Cpn0482 : the arginine binding protein from the periplasm of chlamydia Pneumoniae==
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The line below this paragraph, containing "STRUCTURE_3n26", creates the "Structure Box" on the page.
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<StructureSection load='3n26' size='340' side='right'caption='[[3n26]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3n26]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydia_pneumoniae Chlamydia pneumoniae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3g41 3g41]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N26 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene></td></tr>
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{{STRUCTURE_3n26| PDB=3n26 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n26 OCA], [https://pdbe.org/3n26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n26 RCSB], [https://www.ebi.ac.uk/pdbsum/3n26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n26 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARTJ_CHLPN ARTJ_CHLPN] Probably part of an ABC transporter complex involved in arginine transport. Binds arginine. Interacts with host epithelial cells, suggesting a role in host-cell adhesion during infection.<ref>PMID:20592031</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/3n26_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n26 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We present an interdisciplinary approach that, by incorporating a range of experimental and computational techniques, allows the identification and characterization of functional/immunogenic domains. This approach has been applied to ArtJ, an arginine binding protein whose orthologs in Chlamydiae trachomatis (CT ArtJ) and pneumoniae (CPn ArtJ) are shown to have different immunogenic properties despite a high sequence similarity (60% identity). We have solved the crystallographic structures of CT ArtJ and CPn ArtJ, which are found to display a type-II transporter fold organized in two alpha-beta domains with the arginine-binding region at their interface. Although ArtJ is considered to belong to the periplasm, we found that both domains contain regions exposed on the bacterial surface. Moreover, we show that recombinant ArtJ binds to epithelial cells in vitro, suggesting a role for ArtJ in host-cell adhesion during Chlamydia infection. Experimental epitope mapping and computational analysis of physico-chemical determinants of antibody recognition revealed that immunogenic epitopes reside mainly in the terminal (D1) domain of both CPn and CT ArtJ, while the surface properties of the respective binding-prone regions appear sufficiently different to assume divergent immunogenic behavior. Neutralization assays revealed that sera raised against CPn ArtJ D1 partially reduce both CPn and CT infectivity in vitro, suggesting that functional antibodies directed against this domain may potentially impair chlamydial infectivity. These findings suggest that the approach presented here, combining functional and structure-based analyses of evolutionary-related antigens can be a valuable tool for the identification of cross-species immunogenic epitopes for vaccine development.
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===Cpn0482 : the arginine binding protein from the periplasm of chlamydia Pneumoniae===
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Exploiting antigenic diversity for vaccine design: the Chlamydia ArtJ paradigm.,Soriani M, Petit P, Grifantini R, Petracca R, Gancitano G, Frigimelica E, Nardelli F, Garcia C, Spinelli S, Scarabelli G, Fiorucci S, Affentranger R, Ferrer-Navarro M, Zacharias M, Colombo G, Vuillard L, Daura X, Grandi G J Biol Chem. 2010 Jun 30. PMID:20592031<ref>PMID:20592031</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3n26" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20592031}}, adds the Publication Abstract to the page
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*[[ABC transporter 3D structures|ABC transporter 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20592031 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20592031}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Chlamydia pneumoniae]]
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3N26 is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydophila_pneumoniae Chlamydophila pneumoniae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3g41 3g41]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N26 OCA].
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[[Category: Large Structures]]
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[[Category: Garcia C]]
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==Reference==
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[[Category: Grandi G]]
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<ref group="xtra">PMID:20592031</ref><references group="xtra"/>
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[[Category: Petit P]]
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[[Category: Chlamydophila pneumoniae]]
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[[Category: Soriani M]]
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[[Category: Garcia, C.]]
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[[Category: Vuillard L]]
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[[Category: Grandi, G.]]
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[[Category: MSGP, Marseilles Structural Genomics Program AFMB.]]
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[[Category: Petit, P.]]
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[[Category: Soriani, M.]]
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[[Category: Vuillard, L.]]
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[[Category: Bacabs - eu fp6 programme]]
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[[Category: Marseilles structural genomics program @ afmb]]
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[[Category: Msgp]]
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[[Category: Structural genomic]]
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[[Category: Transport protein]]
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[[Category: Vaccine development]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 15:52:53 2010''
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Current revision

Cpn0482 : the arginine binding protein from the periplasm of chlamydia Pneumoniae

PDB ID 3n26

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