3k3t

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{{Seed}}
 
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[[Image:3k3t.jpg|left|200px]]
 
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==E185A mutant of peptidoglycan hydrolase from Sphingomonas sp. A1==
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The line below this paragraph, containing "STRUCTURE_3k3t", creates the "Structure Box" on the page.
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<StructureSection load='3k3t' size='340' side='right'caption='[[3k3t]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3k3t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingomonas_sp._A1 Sphingomonas sp. A1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K3T FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3k3t| PDB=3k3t | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k3t OCA], [https://pdbe.org/3k3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k3t RCSB], [https://www.ebi.ac.uk/pdbsum/3k3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k3t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B7XH69_9SPHN B7XH69_9SPHN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k3/3k3t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k3t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The flagellar protein FlgJ, a member of glycoside hydrolase family 73, has N- and C-terminal domains that are responsible for flagellar rod assembly and peptidoglycan hydrolysis, respectively. The crystal structure of the C-terminal domain of SPH1045 (SPH1045-C), the FlgJ from Sphingomonas sp. strain A1, showed a long cleft formed by two lobes, alpha and beta. In this study, seven site-specific mutants of residues in the cleft were prepared and analyzed. Enzyme activity was reduced most significantly in mutants E185A and Y281A, followed by E224A. A comparison of the crystal structure of the inactive mutant E185A with that of other related enzymes revealed that Glu185 is structurally reasonable as the proton donor and that Tyr281 is close to Glu185. Glu224 is, however, far from the catalytic site, which is inconsistent with the decreased activity exhibited by E224A. The structural flexibility of Glu224 and its neighboring residues observed in SPH1045-C may indicate that this region is able to change its conformation upon substrate binding. ((c) 2010 WILEY-VCH Verlag GmbH &amp; Co. KGaA, Weinheim).
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===E185A mutant of peptidoglycan hydrolase from Sphingomonas sp. A1===
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Mutational studies of the peptidoglycan hydrolase FlgJ of Sphingomonas sp. strain A1.,Maruyama Y, Ochiai A, Itoh T, Mikami B, Hashimoto W, Murata K J Basic Microbiol. 2010 Jun 28. PMID:20586063<ref>PMID:20586063</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20586063}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3k3t" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20586063 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20586063}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3K3T is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphingomonas_sp._a1 Sphingomonas sp. a1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K3T OCA].
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[[Category: Sphingomonas sp. A1]]
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[[Category: Hashimoto W]]
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==Reference==
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[[Category: Itoh T]]
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<ref group="xtra">PMID:20586063</ref><references group="xtra"/>
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[[Category: Maruyama Y]]
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[[Category: Sphingomonas sp. a1]]
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[[Category: Mikami B]]
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[[Category: Hashimoto, W.]]
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[[Category: Murata K]]
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[[Category: Itoh, T.]]
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[[Category: Ochiai A]]
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[[Category: Maruyama, Y.]]
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[[Category: Mikami, B.]]
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[[Category: Murata, K.]]
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[[Category: Ochiai, A.]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 16:19:02 2010''
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Current revision

E185A mutant of peptidoglycan hydrolase from Sphingomonas sp. A1

PDB ID 3k3t

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