3n1n

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{{Seed}}
 
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[[Image:3n1n.jpg|left|200px]]
 
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==Crystal structure of the complex of type I ribosome inactivating protein with guanine at 2.2A resolution==
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The line below this paragraph, containing "STRUCTURE_3n1n", creates the "Structure Box" on the page.
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<StructureSection load='3n1n' size='340' side='right'caption='[[3n1n]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3n1n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_balsamina Momordica balsamina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N1N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N1N FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GUN:GUANINE'>GUN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_3n1n| PDB=3n1n | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n1n OCA], [https://pdbe.org/3n1n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n1n RCSB], [https://www.ebi.ac.uk/pdbsum/3n1n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n1n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D9J2T9_MOMBA D9J2T9_MOMBA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n1/3n1n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n1n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ribosome inactivating proteins (RIPs) of type 1 are plant toxins that eliminate adenine base selectively from the single stranded loop of rRNA. We report six crystal structures, type 1 RIP from Momordica balsamina (A), three in complexed states with ribose (B), guanine (C) and adenine (D) and two structures of MbRIP-1 when crystallized with adenosine triphosphate (ATP) (E) and 2'-deoxyadenosine triphosphate (2'-dATP) (F). These were determined at 1.67A, 1.60A, 2.20A, 1.70A, 2.07A and 1.90A resolutions respectively. The structures contained, (A) unbound protein molecule, (B) one protein molecule and one ribose sugar, (C) one protein molecule and one guanine base, (D) one protein molecule and one adenine base, (E) one protein molecule and one ATP-product adenine molecule and (F) one protein molecule and one 2'-dATP-product adenine molecule. Three distinct conformations of the side chain of Tyr70 were observed with (i) chi(1)=-66 degrees and chi(2)=165 degrees in structures (A) and (B); (ii) chi(1)=-95 degrees and chi(2)=70 degrees in structures (C), (D) and (E); and (iii) chi(1)=-163 degrees and chi(2)=87 degrees in structure (F). The conformation of Tyr70 in (F) corresponds to the structure of a conformational intermediate. This is the first structure which demonstrates that the slow conversion of DNA substrates by RIPs can be trapped during crystallization.
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===Crystal structure of the complex of type I ribosome inactivating protein with guanine at 2.2A resolution===
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Crystal structures of a type-1 ribosome inactivating protein from Momordica balsamina in the bound and unbound states.,Kushwaha GS, Pandey N, Sinha M, Singh SB, Kaur P, Sharma S, Singh TP Biochim Biophys Acta. 2012 Apr;1824(4):679-91. Epub 2012 Feb 14. PMID:22361570<ref>PMID:22361570</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3n1n" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3N1N is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_balsamina Momordica balsamina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N1N OCA].
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*[[Ribosome inactivating protein 3D structures|Ribosome inactivating protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Momordica balsamina]]
[[Category: Momordica balsamina]]
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[[Category: RRNA N-glycosylase]]
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[[Category: Betzel C]]
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[[Category: Betzel, C.]]
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[[Category: Kaur P]]
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[[Category: Kaur, P.]]
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[[Category: Kushwaha GS]]
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[[Category: Kushwaha, G S.]]
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[[Category: Sharma S]]
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[[Category: Sharma, S.]]
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[[Category: Singh N]]
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[[Category: Singh, N.]]
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[[Category: Singh TP]]
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[[Category: Singh, T P.]]
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[[Category: Sinha M]]
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[[Category: Sinha, M.]]
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[[Category: Hydrolase]]
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[[Category: Rip]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 16:22:41 2010''
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Current revision

Crystal structure of the complex of type I ribosome inactivating protein with guanine at 2.2A resolution

PDB ID 3n1n

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