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2cl8

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[[Image:2cl8.jpg|left|200px]]<br /><applet load="2cl8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cl8, resolution 2.80&Aring;" />
 
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'''DECTIN-1 IN COMPLEX WITH BETA-GLUCAN'''<br />
 
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==About this Structure==
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==Dectin-1 in complex with beta-glucan==
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2CL8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with CA and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Bgc Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CL8 OCA].
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<StructureSection load='2cl8' size='340' side='right'caption='[[2cl8]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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[[Category: Mus musculus]]
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== Structural highlights ==
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[[Category: Single protein]]
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<table><tr><td colspan='2'>[[2cl8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CL8 FirstGlance]. <br>
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[[Category: Brown, G.D.]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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[[Category: Brown, J.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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[[Category: Callaghan, C.A.O.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cl8 OCA], [https://pdbe.org/2cl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cl8 RCSB], [https://www.ebi.ac.uk/pdbsum/2cl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cl8 ProSAT]</span></td></tr>
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[[Category: Gilbert, R.J.C.]]
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</table>
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[[Category: Gordon, S.]]
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== Function ==
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[[Category: Jones, E.Y.]]
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[https://www.uniprot.org/uniprot/CLC7A_MOUSE CLC7A_MOUSE] Lectin that functions as pattern receptor specific for beta-1,3-linked and beta-1,6-linked glucans, such as cell wall constituents from pathogenic bacteria and fungi. Necessary for the TLR2-mediated inflammatory response and for TLR2-mediated activation of NF-kappa-B. Enhances cytokine production in macrophages and dendritic cells. Mediates production of reactive oxygen species in the cell. Mediates phagocytosis of C.albicans conidia. Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation.<ref>PMID:10779524</ref> <ref>PMID:11544516</ref> <ref>PMID:12719479</ref> <ref>PMID:15213161</ref> <ref>PMID:15729357</ref> <ref>PMID:15731053</ref> <ref>PMID:15845454</ref> <ref>PMID:16825490</ref>
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[[Category: Marshall, A.S.J.]]
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== Evolutionary Conservation ==
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[[Category: Siebold, C.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: CA]]
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Check<jmol>
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[[Category: CL]]
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<jmolCheckbox>
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[[Category: beta-glucan]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cl/2cl8_consurf.spt"</scriptWhenChecked>
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[[Category: c-type lectin-like domain]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: carbohydrate]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: ctld]]
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</jmolCheckbox>
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[[Category: dectin-1]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cl8 ConSurf].
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[[Category: fungal recognition]]
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<div style="clear:both"></div>
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[[Category: laminarin]]
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<div style="background-color:#fffaf0;">
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[[Category: receptor]]
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== Publication Abstract from PubMed ==
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The murine molecule dectin-1 (known as the beta-glucan receptor in humans) is an immune cell surface receptor implicated in the immunological defense against fungal pathogens. Sequence analysis has indicated that the dectin-1 extracellular domain is a C-type lectin-like domain, and functional studies have established that it binds fungal beta-glucans. We report several dectin-1 crystal structures, including a high-resolution structure and a 2.8 angstroms resolution structure in which a short soaked natural beta-glucan is trapped in the crystal lattice. In vitro characterization of dectin-1 in the presence of its natural ligand indicates higher-order complex formation between dectin-1 and beta-glucans. These combined structural and biophysical data considerably extend the current knowledge of dectin-1 structure and function, and suggest potential mechanisms of defense against fungal pathogens.
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 19:26:52 2007''
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Structure of the fungal beta-glucan-binding immune receptor dectin-1: implications for function.,Brown J, O'Callaghan CA, Marshall AS, Gilbert RJ, Siebold C, Gordon S, Brown GD, Jones EY Protein Sci. 2007 Jun;16(6):1042-52. Epub 2007 May 1. PMID:17473009<ref>PMID:17473009</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2cl8" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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[[Category: Brown GD]]
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[[Category: Brown J]]
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[[Category: Gilbert RJC]]
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[[Category: Gordon S]]
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[[Category: Jones EY]]
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[[Category: Marshall ASJ]]
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[[Category: O'Callaghan CA]]
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[[Category: Siebold C]]

Current revision

Dectin-1 in complex with beta-glucan

PDB ID 2cl8

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