3hrv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (01:54, 21 November 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:3hrv.png|left|200px]]
 
-
<!--
+
==Crystal structure of TcpA, a Type IV pilin from Vibrio cholerae El Tor biotype==
-
The line below this paragraph, containing "STRUCTURE_3hrv", creates the "Structure Box" on the page.
+
<StructureSection load='3hrv' size='340' side='right'caption='[[3hrv]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[3hrv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HRV FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_3hrv| PDB=3hrv | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hrv OCA], [https://pdbe.org/3hrv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hrv RCSB], [https://www.ebi.ac.uk/pdbsum/3hrv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hrv ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/TCPA_VIBCH TCPA_VIBCH]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hr/3hrv_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hrv ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Type IV pili (T4P) are critical to virulence for Vibrio cholerae and other bacterial pathogens. Among their diverse functions, T4P mediate microcolony formation, which protects the bacteria from host defences and concentrates secreted toxins. The T4P of the two V. cholerae O1 disease biotypes, classical and El Tor, share 81% identity in their TcpA subunits, yet these filaments differ in their interaction patterns as assessed by electron microscopy. To understand the molecular basis for pilus-mediated microcolony formation, we solved a 1.5 A resolution crystal structure of N-terminally truncated El Tor TcpA and compared it with that of classical TcpA. Residues that differ between the two pilins are located on surface-exposed regions of the TcpA subunits. By iteratively changing these non-conserved amino acids in classical TcpA to their respective residues in El Tor TcpA, we identified residues that profoundly affect pilus:pilus interaction patterns and bacterial aggregation. These residues lie on either the protruding d-region of the TcpA subunit or in a cavity between pilin subunits in the pilus filament. Our results support a model whereby pili interact via intercalation of surface protrusions on one filament into depressions between subunits on adjacent filaments as a means to hold V. cholerae cells together in microcolonies.
-
===Crystal structure of TcpA, a Type IV pilin from Vibrio cholerae El Tor biotype===
+
Vibrio cholerae El Tor TcpA crystal structure and mechanism for pilus-mediated microcolony formation.,Lim MS, Ng D, Zong Z, Arvai AS, Taylor RK, Tainer JA, Craig L Mol Microbiol. 2010 Aug;77(3):755-70. Epub 2010 Jun 1. PMID:20545841<ref>PMID:20545841</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
==About this Structure==
+
</div>
-
3HRV is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HRV OCA].
+
<div class="pdbe-citations 3hrv" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
-
[[Category: Arvai, A S.]]
+
[[Category: Arvai AS]]
-
[[Category: Craig, L.]]
+
[[Category: Craig L]]
-
[[Category: Tainer, J A.]]
+
[[Category: Tainer JA]]
-
[[Category: Cell adhesion]]
+
-
[[Category: Colonization]]
+
-
[[Category: Microcolony]]
+
-
[[Category: Pilin]]
+
-
[[Category: Toxin-coregulated pilus]]
+
-
[[Category: Type iv pili]]
+
-
[[Category: Vibrio cholerae]]
+
-
[[Category: Virulence factor]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 21 09:48:47 2010''
+

Current revision

Crystal structure of TcpA, a Type IV pilin from Vibrio cholerae El Tor biotype

PDB ID 3hrv

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3hrv"
Personal tools