3k4q

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{{Seed}}
 
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[[Image:3k4q.png|left|200px]]
 
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==Aspergillus niger Phytase in complex with myo-inositol hexakis sulfate==
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The line below this paragraph, containing "STRUCTURE_3k4q", creates the "Structure Box" on the page.
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<StructureSection load='3k4q' size='340' side='right'caption='[[3k4q]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3k4q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K4Q FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IHS:D-MYO-INOSITOL-HEXASULPHATE'>IHS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_3k4q| PDB=3k4q | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k4q OCA], [https://pdbe.org/3k4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k4q RCSB], [https://www.ebi.ac.uk/pdbsum/3k4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k4q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHYA_ASPNG PHYA_ASPNG] Catalyzes the hydrolysis of inorganic orthophosphate from phytate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/3k4q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k4q ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phytases hydrolyse the phosphomonoesters of phytate (myo-inositol-1,2,3,4,5,6-hexakis phosphate) and thus find uses in plant and animal production through the mobilisation of phosphorus from this source. The structure of partially deglycosylated Aspergillus niger PhyA is presented in apo form and in complex with the potent inhibitor myo-inositol-1,2,3,4,5,6-hexakis sulfate, which by analogy with phytate provides a snapshot of the Michaelis complex. The structure explains the enzyme's preference for the 3'-phosphate of phytate. The apo-and inhibitor-bound forms are similar and no induced-fit mechanism operates. Furthermore the enzyme structure is apparently unaffected by the presence of glycosides on the surface. The new structures of A. niger PhyA are discussed in the context of protein engineering studies aimed at modulating pH preference and stability.
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===Aspergillus niger Phytase in complex with myo-inositol hexakis sulfate===
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The structure of Aspergillus niger phytase PhyA in complex with a phytate mimetic.,Oakley AJ Biochem Biophys Res Commun. 2010 Jul 9;397(4):745-9. Epub 2010 Jun 10. PMID:20541524<ref>PMID:20541524</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3k4q" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20541524}}, adds the Publication Abstract to the page
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*[[Phytase 3D structures|Phytase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20541524 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20541524}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3K4Q is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K4Q OCA].
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==Reference==
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<ref group="xtra">PMID:20541524</ref><references group="xtra"/>
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[[Category: 3-phytase]]
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[[Category: Aspergillus niger]]
[[Category: Aspergillus niger]]
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[[Category: Oakley, A J.]]
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[[Category: Large Structures]]
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[[Category: 3-phosphotase]]
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[[Category: Oakley AJ]]
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[[Category: 37288-11-2]]
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[[Category: 70701-62-1]]
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[[Category: Disulfide bond]]
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[[Category: Glycoprotein]]
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[[Category: Hydrolase]]
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[[Category: Myo-inositol hexakis phosphate phosphohydrolase]]
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[[Category: Myo-inositol hexakis sulfate]]
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[[Category: Phya]]
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[[Category: Phytase]]
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[[Category: Secreted]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 21 09:53:26 2010''
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Current revision

Aspergillus niger Phytase in complex with myo-inositol hexakis sulfate

PDB ID 3k4q

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