2x3y

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{{Seed}}
 
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[[Image:2x3y.png|left|200px]]
 
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==Crystal structure of GmhA from Burkholderia pseudomallei==
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The line below this paragraph, containing "STRUCTURE_2x3y", creates the "Structure Box" on the page.
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<StructureSection load='2x3y' size='340' side='right'caption='[[2x3y]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x3y]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X3Y FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2x3y| PDB=2x3y | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x3y OCA], [https://pdbe.org/2x3y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x3y RCSB], [https://www.ebi.ac.uk/pdbsum/2x3y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x3y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GMHA_BURPS GMHA_BURPS] Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.<ref>PMID:20447408</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x3/2x3y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x3y ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Heptoses are found in the surface polysaccharides of most bacteria, contributing to structures that are essential for virulence and antibiotic resistance. Consequently, the biosynthetic enzymes for these sugars are attractive targets for novel antibiotics. The best characterized biosynthetic enzyme is GmhA, which catalyzes the conversion of sedoheptulose-7-phosphate into D-glycero-D-manno-heptopyranose-7-phosphate, the first step in the biosynthesis of heptose. Here, the structure of GmhA from Burkholderia pseudomallei is reported. This enzyme contains a zinc ion at the heart of its active site: this ion stabilizes the active, closed form of the enzyme and presents coordinating side chains as a potential acid and base to drive catalysis. A complex with the product demonstrates that the enzyme retains activity in the crystal and thus suggests that the closed conformation is catalytically relevant and is an excellent target for the development of therapeutics. A revised mechanism for the action of GmhA is postulated on the basis of this structure and the activity of B. pseudomallei GmhA mutants.
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===CRYSTAL STRUCTURE OF GMHA FROM BURKHOLDERIA PSEUDOMALLEI===
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The structure of sedoheptulose-7-phosphate isomerase from Burkholderia pseudomallei reveals a zinc binding site at the heart of the active site.,Harmer NJ J Mol Biol. 2010 Jul 16;400(3):379-92. Epub 2010 May 4. PMID:20447408<ref>PMID:20447408</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20447408}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x3y" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20447408 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20447408}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Burkholderia pseudomallei K96243]]
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2X3Y is a 8 chains structure with sequences from [http://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X3Y OCA].
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[[Category: Large Structures]]
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[[Category: Harmer NJ]]
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==Reference==
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<ref group="xtra">PMID:20447408</ref><references group="xtra"/>
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[[Category: Burkholderia pseudomallei]]
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[[Category: Harmer, N J.]]
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[[Category: Capsule]]
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[[Category: Capsule biogenesis/degradation]]
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[[Category: Carbohydrate metabolism]]
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[[Category: Isomerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 21 09:54:41 2010''
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Current revision

Crystal structure of GmhA from Burkholderia pseudomallei

PDB ID 2x3y

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