2xhb

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{{Seed}}
 
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[[Image:2xhb.jpg|left|200px]]
 
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==Crystal structure of DNA polymerase from Thermococcus gorgonarius in complex with hypoxanthine-containing DNA==
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The line below this paragraph, containing "STRUCTURE_2xhb", creates the "Structure Box" on the page.
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<StructureSection load='2xhb' size='340' side='right'caption='[[2xhb]], [[Resolution|resolution]] 2.72&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xhb]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_gorgonarius Thermococcus gorgonarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XHB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.72&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_2xhb| PDB=2xhb | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xhb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xhb OCA], [https://pdbe.org/2xhb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xhb RCSB], [https://www.ebi.ac.uk/pdbsum/2xhb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xhb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DPOL_THEGO DPOL_THEGO] In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xh/2xhb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xhb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Archaeal family-B DNA polymerases stall replication on encountering the pro-mutagenic bases uracil and hypoxanthine. This publication describes an X-ray crystal structure of Thermococcus gorgonarius polymerase in complex with a DNA containing hypoxanthine in the single-stranded region of the template, two bases ahead of the primer-template junction. Full details of the specific recognition of hypoxanthine are revealed, allowing a comparison with published data that describe uracil binding. The two bases are recognized by the same pocket, in the N-terminal domain, and make very similar protein-DNA interactions. Specificity for hypoxanthine (and uracil) arises from a combination of polymerase-base hydrogen bonds and shape fit between the deaminated bases and the pocket. The structure with hypoxanthine at position 2 explains the stimulation of the polymerase 3'-5' proofreading exonuclease, observed with deaminated bases at this location. A beta-hairpin element, involved in partitioning the primer strand between the polymerase and exonuclease active sites, inserts between the two template bases at the extreme end of the double-stranded DNA. This denatures the two complementary primer bases and directs the resulting 3' single-stranded extension toward the exonuclease active site. Finally, the relative importance of hydrogen bonding and shape fit in determining selectivity for deaminated bases has been examined using nonpolar isosteres. Affinity for both 2,4-difluorobenzene and fluorobenzimidazole, non-hydrogen bonding shape mimics of uracil and hypoxanthine, respectively, is strongly diminished, suggesting polar protein-base contacts are important. However, residual interaction with 2,4-difluorobenzene is seen, confirming a role for shape recognition.
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===CRYSTAL STRUCTURE OF DNA POLYMERASE FROM THERMOCOCCUS GORGONARIUS IN COMPLEX WITH HYPOXANTHINE-CONTAINING DNA===
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Probing the interaction of archaeal DNA polymerases with deaminated bases using X-ray crystallography and non-hydrogen bonding isosteric base analogues.,Killelea T, Ghosh S, Tan SS, Heslop P, Firbank SJ, Kool ET, Connolly BA Biochemistry. 2010 Jul 13;49(27):5772-81. PMID:20527806<ref>PMID:20527806</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xhb" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20527806}}, adds the Publication Abstract to the page
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20527806 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20527806}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2XHB is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Thermococcus_gorgonarius Thermococcus gorgonarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XHB OCA].
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==Reference==
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<ref group="xtra">PMID:20527806</ref><references group="xtra"/>
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[[Category: DNA-directed DNA polymerase]]
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[[Category: Thermococcus gorgonarius]]
[[Category: Thermococcus gorgonarius]]
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[[Category: Connolly, B A.]]
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[[Category: Connolly BA]]
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[[Category: Firbank, S J.]]
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[[Category: Firbank SJ]]
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[[Category: Ghosh, S.]]
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[[Category: Ghosh S]]
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[[Category: Heslop, P.]]
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[[Category: Heslop P]]
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[[Category: Killelea, T.]]
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[[Category: Killelea T]]
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[[Category: Kool, E T.]]
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[[Category: Kool ET]]
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[[Category: Tan, S S.]]
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[[Category: Tan SS]]
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[[Category: Dna damage]]
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[[Category: Exo minus]]
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[[Category: Exonuclease]]
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[[Category: Replication]]
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[[Category: Transferase-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 21 10:14:55 2010''
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Current revision

Crystal structure of DNA polymerase from Thermococcus gorgonarius in complex with hypoxanthine-containing DNA

PDB ID 2xhb

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