3n0f

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{{Seed}}
 
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[[Image:3n0f.jpg|left|200px]]
 
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==Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens)==
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The line below this paragraph, containing "STRUCTURE_3n0f", creates the "Structure Box" on the page.
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<StructureSection load='3n0f' size='340' side='right'caption='[[3n0f]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3n0f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_tremula_x_Populus_alba Populus tremula x Populus alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N0F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n0f OCA], [https://pdbe.org/3n0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n0f RCSB], [https://www.ebi.ac.uk/pdbsum/3n0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n0f ProSAT]</span></td></tr>
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{{STRUCTURE_3n0f| PDB=3n0f | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ISPS_POPCN ISPS_POPCN] Lyase that catalyzes the formation of isoprene from dimethylallyl diphosphate via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base.<ref>PMID:11506373</ref> <ref>PMID:20624401</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/3n0f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n0f ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The X-ray crystal structure of recombinant PcISPS (isoprene synthase from gray poplar hybrid Populusxcanescens) has been determined at 2.7 A resolution, and the structure of its complex with three Mg(2+) and the unreactive substrate analogue dimethylallyl-S-thiolodiphosphate has been determined at 2.8 A resolution. Analysis of these structures suggests that the generation of isoprene from substrate dimethylallyl diphosphate occurs via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base. This chemical mechanism is responsible for the annual atmospheric emission of 100 Tg of isoprene by terrestrial plant life. Importantly, the PcISPS structure promises to guide future protein engineering studies, potentially leading to hydrocarbon fuels and products that do not rely on traditional petrochemical sources.
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===Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens)===
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Structure of Isoprene Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission.,Koksal M, Zimmer I, Schnitzler JP, Christianson DW J Mol Biol. 2010 Jul 17. PMID:20624401<ref>PMID:20624401</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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3N0F is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Populus_tremula_x_populus_alba Populus tremula x populus alba]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0F OCA].
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<div class="pdbe-citations 3n0f" style="background-color:#fffaf0;"></div>
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[[Category: Isoprene synthase]]
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== References ==
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[[Category: Populus tremula x populus alba]]
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<references/>
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[[Category: Christianson, D W.]]
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__TOC__
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[[Category: Koksal, M.]]
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</StructureSection>
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[[Category: Ddxxd motif]]
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[[Category: Large Structures]]
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[[Category: Hemiterpene synthase]]
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[[Category: Populus tremula x Populus alba]]
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[[Category: Lyase]]
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[[Category: Christianson DW]]
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[[Category: Nse/dte motif]]
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[[Category: Koksal M]]
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[[Category: Terpene cyclase fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 21 10:19:56 2010''
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Current revision

Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens)

PDB ID 3n0f

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