3k0k

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{{Seed}}
 
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[[Image:3k0k.png|left|200px]]
 
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==Crystal Structure of BRCA1 BRCT in complex with a minimal recognition tetrapeptide with a free carboxy C-terminus.==
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The line below this paragraph, containing "STRUCTURE_3k0k", creates the "Structure Box" on the page.
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<StructureSection load='3k0k' size='340' side='right'caption='[[3k0k]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3k0k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K0K FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
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{{STRUCTURE_3k0k| PDB=3k0k | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k0k OCA], [https://pdbe.org/3k0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k0k RCSB], [https://www.ebi.ac.uk/pdbsum/3k0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k0k ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/3k0k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k0k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The tandem BRCT domains of BRCA1 and MDC1 facilitate protein signaling at DNA damage foci through specific interactions with serine-phosphorylated protein partners. The MDC1 BRCT binds pSer-Gln-Glu-Tyr-COO(-) at the C terminus of the histone variant gammaH2AX via direct recognition of the C-terminal carboxylate, while BRCA1 recognizes pSer-X-X-Phe motifs either at C-terminal or internal sites within target proteins. Using fluorescence polarization binding assays, we show that while both BRCTs prefer a free main chain carboxylate at the +3 position, this preference is much more pronounced in MDC1. Crystal structures of BRCA1 and MDC1 bound to tetrapeptide substrates reveal differences in the environment of conserved arginines (Arg1699 in BRCA1 and Arg1933 in MDC1) that determine the relative affinity for peptides with -COO(-) versus -CO-NH(2) termini. A mutation in MDC1 that induces a more BRCA1-like conformation relaxes the binding specificity, allowing the mutant to bind phosphopeptides lacking a -COO(-) terminus.
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===Crystal Structure of BRCA1 BRCT in complex with a minimal recognition tetrapeptide with a free carboxy C-terminus.===
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Comparison of the structures and peptide binding specificities of the BRCT domains of MDC1 and BRCA1.,Campbell SJ, Edwards RA, Glover JN Structure. 2010 Feb 10;18(2):167-76. PMID:20159462<ref>PMID:20159462</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3k0k" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20159462}}, adds the Publication Abstract to the page
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*[[BRCA 3D structures|BRCA 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20159462 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20159462}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3K0K is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K0K OCA].
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==Reference==
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<ref group="xtra">PMID:20159462</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Campbell, S J.]]
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[[Category: Large Structures]]
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[[Category: Edwards, R A.]]
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[[Category: Campbell SJ]]
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[[Category: Glover, J N.]]
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[[Category: Edwards RA]]
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[[Category: Abraxa]]
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[[Category: Glover JN]]
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[[Category: Alternative initiation]]
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[[Category: Brca1]]
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[[Category: Brct domain]]
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[[Category: Cell cycle]]
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[[Category: Disease mutation]]
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[[Category: Dna damage]]
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[[Category: Dna damage response]]
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[[Category: Dna repair]]
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[[Category: Dna-binding]]
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[[Category: Fatty acid biosynthesis]]
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[[Category: Ligase]]
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[[Category: Lipid synthesis]]
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[[Category: Metal-binding]]
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[[Category: Nucleus]]
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[[Category: Phospho peptide interaction]]
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[[Category: Phosphoprotein]]
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[[Category: Protein binding]]
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[[Category: Tumor suppressor]]
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[[Category: Ubl conjugation pathway]]
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[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 28 11:57:44 2010''
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Current revision

Crystal Structure of BRCA1 BRCT in complex with a minimal recognition tetrapeptide with a free carboxy C-terminus.

PDB ID 3k0k

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