3n2k

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{{Seed}}
 
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[[Image:3n2k.png|left|200px]]
 
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==TUBULIN-NSC 613862: RB3 Stathmin-like domain complex==
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The line below this paragraph, containing "STRUCTURE_3n2k", creates the "Structure Box" on the page.
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<StructureSection load='3n2k' size='340' side='right'caption='[[3n2k]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3n2k]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N2K FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=K2N:ETHYL+[(2S)-5-AMINO-2-METHYL-3-PHENYL-1,2-DIHYDROPYRIDO[3,4-B]PYRAZIN-7-YL]CARBAMATE'>K2N</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_3n2k| PDB=3n2k | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n2k OCA], [https://pdbe.org/3n2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n2k RCSB], [https://www.ebi.ac.uk/pdbsum/3n2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n2k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/STMN4_RAT STMN4_RAT] Exhibits microtubule-destabilizing activity.<ref>PMID:15039434</ref> <ref>PMID:12111843</ref> <ref>PMID:15014504</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/3n2k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n2k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Tubulin is able to switch between a straight microtubule-like structure and a curved structure in complex with the stathmin-like domain of the RB3 protein (T2RB3). GTP hydrolysis following microtubule assembly induces protofilament curvature and disassembly. The conformation of the labile tubulin heterodimers is unknown. One important question is whether free GDP-tubulin dimers are straightened by GTP binding, or if GTP-tubulin is also curved and switches into a straight conformation upon assembly. We have obtained insight into the bending flexibility of tubulin by analyzing the interplay of tubulin-stathmin association with the binding of several small molecule inhibitors to the colchicine domain at the tubulin intradimer interface, combining structural and biochemical approaches. The crystal structures of T2RB3 complexes with the chiral R and S isomers of ethyl-5-amino-2-methyl-1,2-dihydro-3-phenylpyrido[3,4-b]pyrazin-7-yl-carba mate, show that their binding site overlaps with colchicine ring A and that both complexes have the same curvature as unliganded T2RB3. The binding of these ligands is incompatible with a straight tubulin structure in microtubules. Analytical ultracentrifugation and binding measurements show that tubulin-stathmin associations (T2RB3, T2Stath) and binding of ligands (R, S, TN-16, or the colchicine analogue MTC) are thermodynamically independent from one another, irrespective of tubulin being bound to GTP or GDP. The fact that the interfacial ligands bind equally well to tubulin dimers or stathmin complexes supports a bent conformation of the free tubulin dimers. It is tempting to speculate that stathmin evolved to recognize curved structures in unassembled and disassembling tubulin, thus regulating microtubule assembly.
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===TUBULIN-NSC 613862: RB3 Stathmin-like domain complex===
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Stathmin and interfacial microtubule inhibitors recognize a naturally curved conformation of tubulin dimers.,Barbier P, Dorleans A, Devred F, Sanz L, Allegro D, Alfonso C, Knossow M, Peyrot V, Andreu JM J Biol Chem. 2010 Jul 30. PMID:20675373<ref>PMID:20675373</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3n2k" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20675373}}, adds the Publication Abstract to the page
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*[[Stathmin-4 3D structures|Stathmin-4 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20675373 is the PubMed ID number.
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*[[Tubulin 3D Structures|Tubulin 3D Structures]]
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== References ==
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{{ABSTRACT_PUBMED_20675373}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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3N2K is a 5 chains structure with sequences from [http://en.wikipedia.org/wiki/Ovis_aries Ovis aries] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N2K OCA].
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[[Category: Large Structures]]
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==Reference==
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<ref group="xtra">PMID:20675373</ref><references group="xtra"/>
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[[Category: Ovis aries]]
[[Category: Ovis aries]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Alfonso, C.]]
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[[Category: Alfonso C]]
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[[Category: Allegro, D.]]
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[[Category: Allegro D]]
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[[Category: Andreu, J M.]]
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[[Category: Andreu JM]]
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[[Category: Barbier, P.]]
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[[Category: Barbier P]]
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[[Category: Devred, F.]]
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[[Category: Devred F]]
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[[Category: Dorleans, A.]]
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[[Category: Dorleans A]]
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[[Category: Knossow, M.]]
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[[Category: Knossow M]]
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[[Category: Peyrot, V.]]
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[[Category: Peyrot V]]
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[[Category: Sanz, L.]]
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[[Category: Sanz L]]
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[[Category: Alpha-tubulin]]
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[[Category: Beta-tubulin]]
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[[Category: Cell cycle]]
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[[Category: Colchicine domain]]
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[[Category: Covalent binding]]
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[[Category: Microtubule]]
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[[Category: Stathmin]]
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[[Category: Tubulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Aug 12 00:13:08 2010''
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Current revision

TUBULIN-NSC 613862: RB3 Stathmin-like domain complex

PDB ID 3n2k

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