2jch

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[[Image:2jch.jpg|left|200px]]<br /><applet load="2jch" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2jch, resolution 2.40&Aring;" />
 
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'''STRUCTURAL AND MECHANISTIC BASIS OF PENICILLIN BINDING PROTEIN INHIBITION BY LACTIVICINS'''<br />
 
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==Overview==
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==Structural and mechanistic basis of penicillin binding protein inhibition by lactivicins==
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beta-lactam antibiotics, including penicillins and cephalosporins, inhibit, penicillin-binding proteins (PBPs), which are essential for bacterial cell, wall biogenesis. Pathogenic bacteria have evolved efficient antibiotic, resistance mechanisms that, in Gram-positive bacteria, include mutations, to PBPs that enable them to avoid beta-lactam inhibition. Lactivicin (LTV;, 1) contains separate cycloserine and gamma-lactone rings and is the only, known natural PBP inhibitor that does not contain a beta-lactam. Here we, show that LTV and a more potent analog, phenoxyacetyl-LTV (PLTV; 2), are, active against clinically isolated, penicillin-resistant Streptococcus, pneumoniae strains. Crystallographic analyses of S. pneumoniae PBP1b, reveal that LTV and PLTV inhibition involves opening of both monocyclic, cycloserine and gamma-lactone rings. In PBP1b complexes, the ring-derived, atoms from LTV and PLTV show a notable structural convergence with those, derived from a complexed cephalosporin (cefotaxime; 3). The structures, imply that derivatives of LTV will be useful in the search for new, antibiotics with activity against beta-lactam-resistant bacteria.
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<StructureSection load='2jch' size='340' side='right'caption='[[2jch]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jch]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_R6 Streptococcus pneumoniae R6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JCH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PL7:(2E)-2-({(2S)-2-CARBOXY-2-[(PHENOXYACETYL)AMINO]ETHOXY}IMINO)PENTANEDIOIC+ACID'>PL7</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jch OCA], [https://pdbe.org/2jch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jch RCSB], [https://www.ebi.ac.uk/pdbsum/2jch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jch ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O70038_STREE O70038_STREE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jc/2jch_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jch ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Beta-lactam antibiotics, including penicillins and cephalosporins, inhibit penicillin-binding proteins (PBPs), which are essential for bacterial cell wall biogenesis. Pathogenic bacteria have evolved efficient antibiotic resistance mechanisms that, in Gram-positive bacteria, include mutations to PBPs that enable them to avoid beta-lactam inhibition. Lactivicin (LTV; 1) contains separate cycloserine and gamma-lactone rings and is the only known natural PBP inhibitor that does not contain a beta-lactam. Here we show that LTV and a more potent analog, phenoxyacetyl-LTV (PLTV; 2), are active against clinically isolated, penicillin-resistant Streptococcus pneumoniae strains. Crystallographic analyses of S. pneumoniae PBP1b reveal that LTV and PLTV inhibition involves opening of both monocyclic cycloserine and gamma-lactone rings. In PBP1b complexes, the ring-derived atoms from LTV and PLTV show a notable structural convergence with those derived from a complexed cephalosporin (cefotaxime; 3). The structures imply that derivatives of LTV will be useful in the search for new antibiotics with activity against beta-lactam-resistant bacteria.
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==About this Structure==
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Structural and mechanistic basis of penicillin-binding protein inhibition by lactivicins.,Macheboeuf P, Fischer DS, Brown T Jr, Zervosen A, Luxen A, Joris B, Dessen A, Schofield CJ Nat Chem Biol. 2007 Sep;3(9):565-9. Epub 2007 Aug 5. PMID:17676039<ref>PMID:17676039</ref>
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2JCH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae_r6 Streptococcus pneumoniae r6] with SO4, CL, EDO and PL7 as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Pl7 Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:So4 Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Cl Binding Site For Chain A'>AC3</scene> and <scene name='pdbsite=AC4:Edo Binding Site For Chain A'>AC4</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JCH OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural and mechanistic basis of penicillin-binding protein inhibition by lactivicins., Macheboeuf P, Fischer DS, Brown T Jr, Zervosen A, Luxen A, Joris B, Dessen A, Schofield CJ, Nat Chem Biol. 2007 Aug 5;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17676039 17676039]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2jch" style="background-color:#fffaf0;"></div>
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[[Category: Streptococcus pneumoniae r6]]
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[[Category: Brown, T.J.]]
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[[Category: Dessen, A.]]
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[[Category: Fisher, D.S.]]
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[[Category: Joris, B.]]
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[[Category: Luxen, A.]]
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[[Category: Macheboeuf, P.]]
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[[Category: Schofield, C.J.]]
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[[Category: Zervosen, A.]]
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[[Category: CL]]
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[[Category: EDO]]
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[[Category: PL7]]
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[[Category: SO4]]
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[[Category: binding protein]]
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[[Category: cell wall]]
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[[Category: drug-binding protein]]
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[[Category: gamma lactam antibiotics]]
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[[Category: peptidoglycan]]
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[[Category: peptidoglycan synthesis multifunctional enzyme]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 20:00:30 2007''
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==See Also==
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*[[Penicillin-binding protein 3D structures|Penicillin-binding protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptococcus pneumoniae R6]]
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[[Category: Brown TJ]]
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[[Category: Dessen A]]
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[[Category: Fisher DS]]
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[[Category: Joris B]]
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[[Category: Luxen A]]
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[[Category: Macheboeuf P]]
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[[Category: Schofield CJ]]
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[[Category: Zervosen A]]

Current revision

Structural and mechanistic basis of penicillin binding protein inhibition by lactivicins

PDB ID 2jch

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