2xo6
From Proteopedia
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(New page: '''Unreleased structure''' The entry 2xo6 is ON HOLD until Paper Publication Authors: HICKMAN, A.B., JAMES, J.A., BARABAS, O., PASTERNAK, C., TON-HOANG, B., CHANDLER, M., SOMMER, S., DY...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE Y132F MUTANT COMPLEXED WITH LEFT END RECOGNITION AND CLEAVAGE SITE== | |
| + | <StructureSection load='2xo6' size='340' side='right'caption='[[2xo6]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2xo6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XO6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xo6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xo6 OCA], [https://pdbe.org/2xo6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xo6 RCSB], [https://www.ebi.ac.uk/pdbsum/2xo6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xo6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DRA2A_DEIRA DRA2A_DEIRA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Bacterial insertion sequences (ISs) from the IS200/IS605 family encode the smallest known DNA transposases and mobilize through single-stranded DNA transposition. Transposition by one particular family member, ISDra2 from Deinococcus radiodurans, is dramatically stimulated upon massive gamma irradiation. We have determined the crystal structures of four ISDra2 transposase/IS end complexes; combined with in vivo activity assays and fluorescence anisotropy binding measurements, these have revealed the molecular basis of strand discrimination and transposase action. The structures also show that previously established structural rules of target site recognition that allow different specific sequences to be targeted are only partially conserved among family members. Furthermore, we have captured a fully assembled active site including the scissile phosphate bound by a divalent metal ion cofactor (Cd(2+)) that supports DNA cleavage. Finally, the observed active site rearrangements when the transposase binds a metal ion in which it is inactive provide a clear rationale for metal ion specificity. | ||
| - | + | DNA recognition and the precleavage state during single-stranded DNA transposition in D. radiodurans.,Hickman AB, James JA, Barabas O, Pasternak C, Ton-Hoang B, Chandler M, Sommer S, Dyda F EMBO J. 2010 Oct 1. PMID:20890269<ref>PMID:20890269</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 2xo6" style="background-color:#fffaf0;"></div> | ||
| - | + | ==See Also== | |
| + | *[[Transposase 3D structures|Transposase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Deinococcus radiodurans]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Barabas O]] | ||
| + | [[Category: Chandler M]] | ||
| + | [[Category: Dyda F]] | ||
| + | [[Category: Hickman AB]] | ||
| + | [[Category: James JA]] | ||
| + | [[Category: Pasternak C]] | ||
| + | [[Category: Sommer S]] | ||
| + | [[Category: Ton-Hoang B]] | ||
Current revision
DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE Y132F MUTANT COMPLEXED WITH LEFT END RECOGNITION AND CLEAVAGE SITE
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