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3nvn
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Molecular mechanism of guidance cue recognition== | |
| + | <StructureSection load='3nvn' size='340' side='right' caption='[[3nvn]], [[Resolution|resolution]] 2.26Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3nvn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ectromelia_mousepox_virus Ectromelia mousepox virus] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NVN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NVN FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3nvx|3nvx]], [[3nvq|3nvq]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SEMA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12643 Ectromelia mousepox virus]), PLXNC1, VESPR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nvn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nvn OCA], [http://pdbe.org/3nvn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nvn RCSB], [http://www.ebi.ac.uk/pdbsum/3nvn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nvn ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/SEMA_ECTVM SEMA_ECTVM]] Acts as a semaphorin-like protein and binds to host plexin C1 receptor. May alter the movement of plexin C1-expressing cells including dendritic cells, monocytes, or granulocytes in the proximity of infected cells. May also regulate host cell cytoskeleton of neighboring cells to improve viral infection.<ref>PMID:15611227</ref> <ref>PMID:15657950</ref> [[http://www.uniprot.org/uniprot/PLXC1_HUMAN PLXC1_HUMAN]] Receptor for SEMA7A, for smallpox semaphorin A39R, vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8 (By similarity).<ref>PMID:20727575</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nv/3nvn_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nvn ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Repulsive signaling by Semaphorins and Plexins is crucial for the development and homeostasis of the nervous, immune, and cardiovascular systems. Sema7A acts as both an immune and a neural Semaphorin through PlexinC1, and A39R is a Sema7A mimic secreted by smallpox virus. We report the structures of Sema7A and A39R complexed with the Semaphorin-binding module of PlexinC1. Both structures show two PlexinC1 molecules symmetrically bridged by Semaphorin dimers, in which the Semaphorin and PlexinC1 beta propellers interact in an edge-on, orthogonal orientation. Both binding interfaces are dominated by the insertion of the Semaphorin's 4c-4d loop into a deep groove in blade 3 of the PlexinC1 propeller. A39R appears to achieve Sema7A mimicry by preserving key Plexin-binding determinants seen in the mammalian Sema7A complex that have evolved to achieve higher affinity binding to the host-derived PlexinC1. The complex structures support a conserved Semaphorin-Plexin recognition mode and suggest that Plexins are activated by dimerization. | ||
| - | + | Structural basis of semaphorin-plexin recognition and viral mimicry from Sema7A and A39R complexes with PlexinC1.,Liu H, Juo ZS, Shim AH, Focia PJ, Chen X, Garcia KC, He X Cell. 2010 Sep 3;142(5):749-61. Epub 2010 Aug 19. PMID:20727575<ref>PMID:20727575</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3nvn" style="background-color:#fffaf0;"></div> | ||
| - | + | ==See Also== | |
| + | *[[Plexin|Plexin]] | ||
| + | *[[Semaphorin|Semaphorin]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Ectromelia mousepox virus]] | ||
| + | [[Category: Human]] | ||
| + | [[Category: Chen, X]] | ||
| + | [[Category: Focia, P]] | ||
| + | [[Category: Garcia, C]] | ||
| + | [[Category: He, X]] | ||
| + | [[Category: Juo, Z]] | ||
| + | [[Category: Liu, H]] | ||
| + | [[Category: Shim, A]] | ||
| + | [[Category: Beta-propeller]] | ||
| + | [[Category: Signaling]] | ||
| + | [[Category: Viral protein-signaling protein complex]] | ||
Current revision
Molecular mechanism of guidance cue recognition
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