3odv
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 3odv is ON HOLD Authors: Pentelute, B. L., Mandal, K., Gates, Z. P., Sawaya, M. R., Yeates, T. O., Kent, S. B. H. Description: X-ray structure of k...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==X-ray structure of kaliotoxin by racemic protein crystallography== | |
| + | <StructureSection load='3odv' size='340' side='right'caption='[[3odv]], [[Resolution|resolution]] 0.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3odv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Androctonus_mauritanicus_mauritanicus Androctonus mauritanicus mauritanicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ODV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ODV FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.95Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=TFA:TRIFLUOROACETIC+ACID'>TFA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3odv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3odv OCA], [https://pdbe.org/3odv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3odv RCSB], [https://www.ebi.ac.uk/pdbsum/3odv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3odv ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/KAX31_ANDMA KAX31_ANDMA] Potent inhibitor of large conductance calcium-activated potassium channels (BK-Ca). Also binds to the dendrotoxin sensitive voltage-dependent potassium channel. It appears to block channel activity by a simple bimolecular inhibition process. Induces a transient period of fast flickering in the channel openings, followed by an almost complete blockade of the channel. Its binding affinity to rat brain synaptosomes is 5-fold higher than this of KTX-3. Binding of the toxin to the channel is associated with significant structural rearrangements in both molecules. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Here we report the total synthesis of kaliotoxin by 'one pot' native chemical ligation of three synthetic peptides. A racemic mixture of d- and l-kaliotoxin synthetic protein molecules gave crystals in the centrosymmetric space group P1[combining macron] that diffracted to atomic-resolution (0.95 A), enabling the X-ray structure of kaliotoxin to be determined by direct methods. | ||
| - | + | Total chemical synthesis and X-ray structure of kaliotoxin by racemic protein crystallography.,Pentelute BL, Mandal K, Gates ZP, Sawaya MR, Yeates TO, Kent SB Chem Commun (Camb). 2010 Sep 28. PMID:20877851<ref>PMID:20877851</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 3odv" style="background-color:#fffaf0;"></div> | ||
| - | + | ==See Also== | |
| + | *[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Androctonus mauritanicus mauritanicus]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Gates ZP]] | ||
| + | [[Category: Kent SBH]] | ||
| + | [[Category: Mandal K]] | ||
| + | [[Category: Pentelute BL]] | ||
| + | [[Category: Sawaya MR]] | ||
| + | [[Category: Yeates TO]] | ||
Current revision
X-ray structure of kaliotoxin by racemic protein crystallography
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