3nnj

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{{Seed}}
 
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[[Image:3nnj.png|left|200px]]
 
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==Halogenase domain from CurA module (apo Hal)==
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The line below this paragraph, containing "STRUCTURE_3nnj", creates the "Structure Box" on the page.
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<StructureSection load='3nnj' size='340' side='right'caption='[[3nnj]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3nnj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NNJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NNJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.601&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3nnj| PDB=3nnj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nnj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nnj OCA], [https://pdbe.org/3nnj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nnj RCSB], [https://www.ebi.ac.uk/pdbsum/3nnj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nnj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6DNF2_9CYAN Q6DNF2_9CYAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nn/3nnj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nnj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The CurA halogenase (Hal) catalyzes a cryptic chlorination leading to cyclopropane ring formation in the synthesis of the natural product curacin A. Hal belongs to a family of enzymes that use Fe(2+), O(2) and alpha-ketoglutarate (alphaKG) to perform a variety of halogenation reactions in natural product biosynthesis. Crystal structures of the enzyme in five ligand states reveal strikingly different open and closed conformations dependent on alphaKG binding. The open form represents ligand-free enzyme, preventing substrate from entering the active site until both alphaKG and chloride are bound, while the closed form represents the holoenzyme with alphaKG and chloride coordinated to iron. Candidate amino acid residues involved in substrate recognition were identified by site-directed mutagenesis. These new structures provide direct evidence of a conformational switch driven by alphaKG leading to chlorination of an early pathway intermediate.
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===Halogenase domain from CurA module (apo Hal)===
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Conformational switch triggered by alpha-ketoglutarate in a halogenase of curacin A biosynthesis.,Khare D, Wang B, Gu L, Razelun J, Sherman DH, Gerwick WH, Hakansson K, Smith JL Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14099-104. Epub 2010 Jul 26. PMID:20660778<ref>PMID:20660778</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20660778}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3nnj" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20660778 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20660778}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3NNJ is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NNJ OCA].
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==Reference==
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<ref group="xtra">PMID:20660778</ref><references group="xtra"/>
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[[Category: Lyngbya majuscula]]
[[Category: Lyngbya majuscula]]
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[[Category: Khare, D.]]
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[[Category: Khare D]]
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[[Category: Smith, J L.]]
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[[Category: Smith JL]]
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[[Category: Biosynthetic protein]]
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[[Category: Catalyzes a cryptic chlorination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 25 08:26:10 2010''
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Current revision

Halogenase domain from CurA module (apo Hal)

PDB ID 3nnj

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OCA

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