3mfk

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{{Seed}}
 
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[[Image:3mfk.png|left|200px]]
 
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==Ets1 complex with stromelysin-1 promoter DNA==
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The line below this paragraph, containing "STRUCTURE_3mfk", creates the "Structure Box" on the page.
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<StructureSection load='3mfk' size='340' side='right'caption='[[3mfk]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3mfk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3MFK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3mfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mfk OCA], [https://pdbe.org/3mfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3mfk RCSB], [https://www.ebi.ac.uk/pdbsum/3mfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3mfk ProSAT]</span></td></tr>
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{{STRUCTURE_3mfk| PDB=3mfk | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ETS1_HUMAN ETS1_HUMAN] Transcription factor.<ref>PMID:10698492</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mf/3mfk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3mfk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ets1 is a member of the Ets family of transcription factors. Ets1 is autoinhibited and its activation requires heterodimerization with a partner protein or DNA-mediated homodimerization for cooperative DNA binding. In the latter case, Ets1 molecules bind to palindromic sequences in which two Ets-binding sites (EBS) are separated by four base pairs, for example in the promoters of stromelysin-1 and p53. Interestingly, counteraction of autoinhibition requires the autoinhibitory region encoded by exon VII of the gene. The structural basis for the requirement of autoinhibitory sequences for Ets1 binding to palindromic EBS still remains unresolved. Here we report the crystal structure of two Ets1 molecules bound to an EBS palindrome of the stromelysin-1 promoter DNA, providing a plausible explanation for the requirement of exon VII-encoded sequences for Ets1 cooperative DNA binding. The proposed mechanism was verified both in vitro by surface plasmon resonance and in vivo by transcription-based assays.
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===Ets1 complex with stromelysin-1 promoter DNA===
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Structural basis of Ets1 cooperative binding to palindromic sequences on stromelysin-1 promoter DNA.,Babayeva ND, Wilder PJ, Shiina M, Mino K, Desler M, Ogata K, Rizzino A, Tahirov TH Cell Cycle. 2010 Aug;9(15):3054-62. Epub 2010 Aug 5. PMID:20686355<ref>PMID:20686355</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3mfk" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20686355}}, adds the Publication Abstract to the page
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*[[Ets1|Ets1]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20686355 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20686355}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3MFK is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MFK OCA].
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==Reference==
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<ref group="xtra">PMID:20686355</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Babayeva, N D.]]
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[[Category: Large Structures]]
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[[Category: Mino, K.]]
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[[Category: Babayeva ND]]
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[[Category: Tahirov, T H.]]
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[[Category: Mino K]]
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[[Category: Ets domain]]
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[[Category: Tahirov TH]]
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[[Category: Ets1]]
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[[Category: Protein-dna complex]]
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[[Category: Stromelysin-1]]
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[[Category: Transcription-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 1 09:15:05 2010''
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Current revision

Ets1 complex with stromelysin-1 promoter DNA

PDB ID 3mfk

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OCA

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