3nqu

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{{Seed}}
 
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[[Image:3nqu.png|left|200px]]
 
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==Crystal structure of partially trypsinized (CENP-A/H4)2 heterotetramer==
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The line below this paragraph, containing "STRUCTURE_3nqu", creates the "Structure Box" on the page.
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<StructureSection load='3nqu' size='340' side='right'caption='[[3nqu]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3nqu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NQU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3nqu| PDB=3nqu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nqu OCA], [https://pdbe.org/3nqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nqu RCSB], [https://www.ebi.ac.uk/pdbsum/3nqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nqu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/H4_HUMAN H4_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nq/3nqu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nqu ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Centromeres are specified epigenetically, and the histone H3 variant CENP-A is assembled into the chromatin of all active centromeres. Divergence from H3 raises the possibility that CENP-A generates unique chromatin features to mark physically centromere location. Here we report the crystal structure of a subnucleosomal heterotetramer, human (CENP-A-H4)(2), that reveals three distinguishing properties encoded by the residues that comprise the CENP-A targeting domain (CATD; ref. 2): (1) a CENP-A-CENP-A interface that is substantially rotated relative to the H3-H3 interface; (2) a protruding loop L1 of the opposite charge as that on H3; and (3) strong hydrophobic contacts that rigidify the CENP-A-H4 interface. Residues involved in the CENP-A-CENP-A rotation are required for efficient incorporation into centromeric chromatin, indicating specificity for an unconventional nucleosome shape. DNA topological analysis indicates that CENP-A-containing nucleosomes are octameric with conventional left-handed DNA wrapping, in contrast to other recent proposals. Our results indicate that CENP-A marks centromere location by restructuring the nucleosome from within its folded histone core.
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===Crystal structure of partially trypsinized (CENP-A/H4)2 heterotetramer===
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The structure of (CENP-A-H4)(2) reveals physical features that mark centromeres.,Sekulic N, Bassett EA, Rogers DJ, Black BE Nature. 2010 Aug 25. PMID:20739937<ref>PMID:20739937</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3nqu" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20739937}}, adds the Publication Abstract to the page
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*[[Centromere protein 3D structure|Centromere protein 3D structure]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20739937 is the PubMed ID number.
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*[[Histone 3D structures|Histone 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_20739937}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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3NQU is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NQU OCA].
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==Reference==
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<ref group="xtra">PMID:20739937</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Black, B E.]]
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[[Category: Large Structures]]
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[[Category: Sekulic, N.]]
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[[Category: Black BE]]
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[[Category: Alpha helix]]
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[[Category: Sekulic N]]
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[[Category: Centromere]]
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[[Category: Dna binding protein]]
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[[Category: Histone fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Sep 10 13:30:07 2010''
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Current revision

Crystal structure of partially trypsinized (CENP-A/H4)2 heterotetramer

PDB ID 3nqu

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