2xi9

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{{Seed}}
 
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[[Image:2xi9.png|left|200px]]
 
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==Pilus-presented adhesin, Spy0125 (Cpa), P1 form==
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The line below this paragraph, containing "STRUCTURE_2xi9", creates the "Structure Box" on the page.
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<StructureSection load='2xi9' size='340' side='right'caption='[[2xi9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xi9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XI9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XI9 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2xi9| PDB=2xi9 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xi9 OCA], [https://pdbe.org/2xi9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xi9 RCSB], [https://www.ebi.ac.uk/pdbsum/2xi9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xi9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8GRA2_STRPY Q8GRA2_STRPY]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xi/2xi9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xi9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many bacterial pathogens present adhesins at the tips of long macromolecular filaments known as pili that are often important virulence determinants. Very little is known about how pili presented by Gram positive pathogens mediate host cell binding. The crystal structure of a pilus adhesin from the important human pathogen Streptococcus pyogenes reveals an internal thioester bond formed between the side chains of a cysteine and a glutamine residue. The presence of the thioester was verified using UV-vis spectroscopy and mass spectrometry. This unusual bond has only previously been observed in thioester domains of complement and complement-like proteins, where it is used to form covalent attachment to target molecules. The structure also reveals two intramolecular isopeptide bonds, one of these formed through a Lys/Asp residue pair, that are strategically positioned to confer protein stability. Removal of the internal thioester by allele-replacement mutagenesis in S. pyogenes severely compromises bacterial adhesion to model host cells. Whilst current paradigms of bacterial/host cell interaction envisage strong non-covalent interactions, the present study suggests cell adhesion could also involve covalent bonds.
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===PILUS-PRESENTED ADHESIN, SPY0125 (CPA), P1 FORM===
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A highly unusual thioester bond in a pilus adhesin is required for efficient host cell interaction.,Pointon JA, Smith WD, Saalbach G, Crow A, Kehoe MA, Banfield MJ J Biol Chem. 2010 Aug 19. PMID:20729215<ref>PMID:20729215</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20729215}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2xi9" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20729215 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20729215}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2XI9 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XI9 OCA].
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==Reference==
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<ref group="xtra">PMID:20729215</ref><references group="xtra"/>
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[[Category: Streptococcus pyogenes]]
[[Category: Streptococcus pyogenes]]
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[[Category: Banfield, M J.]]
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[[Category: Banfield MJ]]
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[[Category: Crow, A.]]
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[[Category: Crow A]]
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[[Category: Kehoe, M A.]]
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[[Category: Kehoe MA]]
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[[Category: Pointon, J A.]]
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[[Category: Pointon JA]]
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[[Category: Saalbach, G.]]
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[[Category: Saalbach G]]
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[[Category: Smith, W D.]]
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[[Category: Smith WD]]
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[[Category: Cell adhesion]]
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[[Category: Gram positive pilus]]
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[[Category: Internal thioester]]
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[[Category: Intramolecular isopeptide bond]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Sep 10 13:36:55 2010''
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Current revision

Pilus-presented adhesin, Spy0125 (Cpa), P1 form

PDB ID 2xi9

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