3iso

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{{Seed}}
 
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[[Image:3iso.jpg|left|200px]]
 
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==Crystal structure of 26 kDa GST of Clonorchis sinensis in P3221 symmetry==
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The line below this paragraph, containing "STRUCTURE_3iso", creates the "Structure Box" on the page.
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<StructureSection load='3iso' size='340' side='right'caption='[[3iso]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3iso]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clonorchis_sinensis Clonorchis sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ISO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ISO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3iso| PDB=3iso | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iso OCA], [https://pdbe.org/3iso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iso RCSB], [https://www.ebi.ac.uk/pdbsum/3iso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iso ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q25595_CLOSI Q25595_CLOSI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/is/3iso_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3iso ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glutathione S-transferases (GSTs) are multifunctional enzymes that are used as fusion tags on recombinant proteins in mammalian and Escherichia coli expression systems. We recently found that the Schistosoma japonicum GST (SjGST) displays weak Ni(2+) ion binding affinity. Glu26 and His79 were assumed to be its Ni(2+) binding sites based on the structure of the 26-kDa Clonorchis sinensis GST. To enhance SjGST Ni(2+) binding affinity, Glu26 was mutated to His. SjGST-E26H was expressed and purified at a high concentration of imidazole to a higher purity than wild type SjGST. In addition, human biotin protein ligase fused to SjGST-E26H was purified with a immobilized Ni affinity column.
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===Crystal structure of 26 kDa GST of Clonorchis sinensis in P3221 symmetry===
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A histidine substitution confers metal binding affinity to a Schistosoma japonicum Glutathione S-transferase.,Han YH, Seo HA, Kim GH, Lee CK, Kang YK, Ryu KH, Chung YJ Protein Expr Purif. 2010 Sep;73(1):74-7. Epub 2010 Mar 27. PMID:20347989<ref>PMID:20347989</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20347989}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3iso" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20347989 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20347989}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3ISO is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Clonorchis_sinensis Clonorchis sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ISO OCA].
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==Reference==
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<ref group="xtra">PMID:20347989</ref><references group="xtra"/>
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[[Category: Clonorchis sinensis]]
[[Category: Clonorchis sinensis]]
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[[Category: Chung, Y J.]]
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[[Category: Large Structures]]
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[[Category: Han, Y H.]]
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[[Category: Chung YJ]]
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[[Category: Kim, G H.]]
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[[Category: Han YH]]
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[[Category: Seo, H A.]]
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[[Category: Kim GH]]
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[[Category: Gst]]
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[[Category: Seo HA]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Sep 10 13:56:43 2010''
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Current revision

Crystal structure of 26 kDa GST of Clonorchis sinensis in P3221 symmetry

PDB ID 3iso

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OCA

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