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3nct

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{{Seed}}
 
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[[Image:3nct.png|left|200px]]
 
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==X-ray crystal structure of the bacterial conjugation factor PsiB, a negative regulator of reca==
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The line below this paragraph, containing "STRUCTURE_3nct", creates the "Structure Box" on the page.
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<StructureSection load='3nct' size='340' side='right'caption='[[3nct]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3nct]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NCT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3nct| PDB=3nct | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nct OCA], [https://pdbe.org/3nct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nct RCSB], [https://www.ebi.ac.uk/pdbsum/3nct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nct ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PSIB1_ECOLI PSIB1_ECOLI] In contrast to PsiB protein of plasmid R6-5, this protein is unable to prevent SOS induction for potentially two reasons: less activity and/or insufficient expression.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nc/3nct_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nct ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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During bacterial conjugation, genetic material from one cell is transferred to another as single-stranded DNA. The introduction of single-stranded DNA into the recipent cell would ordinarily trigger a potentially deleterious transcriptional response called SOS, which is initiated by RecA protein filaments formed on the DNA. During F plasmid conjugation, however, the SOS response is suppressed by PsiB, an F-plasmid-encoded protein that binds and sequesters free RecA to prevent filament formation. Among the many characterized RecA modulator proteins, PsiB is unique in using sequestration as an inhibitory mechanism. We describe the crystal structure of PsiB from the Escherichia coli F plasmid. The stucture of PsiB is surprisingly similar to CapZ, a eukaryotic actin filament capping protein. Structure-directed neutralization of electronegative surfaces on PsiB abrogates RecA inhibition whereas neutralization of an electropositive surface element enhances PsiB inhibition of RecA. Together, these studies provide a first molecular view of PsiB and highlight its use as a reagent in studies of RecA activity.
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===X-ray crystal structure of the bacterial conjugation factor PsiB, a negative regulator of reca===
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X-ray crystal structure of the bacterial conjugation factor PsiB, a negative regulator of RecA.,Petrova V, Satyshur KA, George NP, McCaslin D, Cox MM, Keck JL J Biol Chem. 2010 Jul 21. PMID:20659894<ref>PMID:20659894</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20659894}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3nct" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20659894 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20659894}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3NCT is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NCT OCA].
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==Reference==
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<ref group="xtra">PMID:20659894</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Cox, M M.]]
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[[Category: Large Structures]]
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[[Category: George, N P.]]
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[[Category: Cox MM]]
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[[Category: Keck, J L.]]
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[[Category: George NP]]
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[[Category: McCaslin, D.]]
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[[Category: Keck JL]]
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[[Category: Petrova, V.]]
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[[Category: McCaslin D]]
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[[Category: Satyshur, K A.]]
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[[Category: Petrova V]]
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[[Category: Chaperone]]
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[[Category: Satyshur KA]]
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[[Category: Dna binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 15 10:18:36 2010''
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Current revision

X-ray crystal structure of the bacterial conjugation factor PsiB, a negative regulator of reca

PDB ID 3nct

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