3ohr

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{{Seed}}
 
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[[Image:3ohr.jpg|left|200px]]
 
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==Crystal structure of fructokinase from bacillus subtilis complexed with ADP==
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The line below this paragraph, containing "STRUCTURE_3ohr", creates the "Structure Box" on the page.
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<StructureSection load='3ohr' size='340' side='right' caption='[[3ohr]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ohr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3epq 3epq]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OHR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OHR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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-->
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3ohr| PDB=3ohr | SCENE= }}
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3lm9|3lm9]], [[1xc3|1xc3]]</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gmuE, ydhR, BSU05860 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructokinase Fructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.4 2.7.1.4] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ohr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ohr OCA], [http://pdbe.org/3ohr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ohr RCSB], [http://www.ebi.ac.uk/pdbsum/3ohr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ohr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/SCRK_BACSU SCRK_BACSU]] Seems to be involved in the degradation of glucomannan.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/3ohr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ohr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The main pathway of bacterial sugar phosphorylation utilizes specific phosphoenolpyruvate phosphotransferase system (PTS) enzymes. In addition to the classic PTS system, a PTS-independent secondary system has been described in which nucleotide-dependent sugar kinases are used for monosaccharide phosphorylation. Fructokinase (FK), which phosphorylates d-fructose with ATP as a cofactor, has been shown to be a member of this secondary system. Bioinformatic analysis has shown that FK is a member of the "ROK" (bacterial Repressors, uncharacterized Open reading frames, and sugar Kinases) sequence family. In this study, we report the crystal structures of ROK FK from Bacillus subtilis (YdhR) (a) apo and in the presence of (b) ADP and (c) ADP/d-fructose. All structures show that YdhR is a homodimer with a monomer composed of two similar alpha/beta domains forming a large cleft between domains that bind ADP and D-fructose. Enzymatic activity assays support YdhR function as an ATP-dependent fructose kinase.
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===Crystal structure of fructokinase from bacillus subtilis complexed with ADP===
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Structural studies of ROK fructokinase YdhR from Bacillus subtilis: insights into substrate binding and fructose specificity.,Nocek B, Stein AJ, Jedrzejczak R, Cuff ME, Li H, Volkart L, Joachimiak A J Mol Biol. 2011 Feb 18;406(2):325-42. Epub 2010 Dec 23. PMID:21185308<ref>PMID:21185308</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ohr" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3OHR is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OHR OCA].
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*[[Fructokinase|Fructokinase]]
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[[Category: Bacillus subtilis]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bacillus globigii migula 1900]]
[[Category: Fructokinase]]
[[Category: Fructokinase]]
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[[Category: Cuff, M.]]
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[[Category: Cuff, M]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak, A]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Structural genomic]]
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[[Category: Nocek, B.]]
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[[Category: Nocek, B]]
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[[Category: Volkart, L.]]
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[[Category: Volkart, L]]
[[Category: Adp binding]]
[[Category: Adp binding]]
[[Category: D-fructose binding]]
[[Category: D-fructose binding]]
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[[Category: Fructokinase]]
 
[[Category: Mcsg]]
[[Category: Mcsg]]
[[Category: Metal dependent]]
[[Category: Metal dependent]]
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[[Category: Midwest center for structural genomic]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Reductive methylation]]
[[Category: Reductive methylation]]
[[Category: Rok family]]
[[Category: Rok family]]
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[[Category: Structural genomic]]
 
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 15 10:55:41 2010''
 

Current revision

Crystal structure of fructokinase from bacillus subtilis complexed with ADP

3ohr, resolution 1.66Å

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