3eoy

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{{Seed}}
 
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[[Image:3eoy.png|left|200px]]
 
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==Structure of Reovirus sigma1 in Complex with Its Receptor Junctional Adhesion Molecule-A==
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The line below this paragraph, containing "STRUCTURE_3eoy", creates the "Structure Box" on the page.
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<StructureSection load='3eoy' size='340' side='right'caption='[[3eoy]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3eoy]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_Dearing Mammalian orthoreovirus 3 Dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EOY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3eoy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3eoy OCA], [https://pdbe.org/3eoy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3eoy RCSB], [https://www.ebi.ac.uk/pdbsum/3eoy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3eoy ProSAT]</span></td></tr>
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{{STRUCTURE_3eoy| PDB=3eoy | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SIGM1_REOVD SIGM1_REOVD] Fiber-like molecule that attaches the virion to the host cell membrane by binding to the primary receptor F11R/JAM-A and to sialic acid containing proteins (coreceptor). The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/eo/3eoy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3eoy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Viral attachment to specific host receptors is the first step in viral infection and serves an essential function in the selection of target cells. Mammalian reoviruses are highly useful experimental models for studies of viral pathogenesis and show promise as vectors for oncolytics and vaccines. Reoviruses engage cells by binding to carbohydrates and the immunoglobulin superfamily member, junctional adhesion molecule-A (JAM-A). JAM-A exists at the cell surface as a homodimer formed by extensive contacts between its N-terminal immunoglobulin-like domains. We report the crystal structure of reovirus attachment protein sigma1 in complex with a soluble form of JAM-A. The sigma1 protein disrupts the JAM-A dimer, engaging a single JAM-A molecule via virtually the same interface that is used for JAM-A homodimerization. Thus, reovirus takes advantage of the adhesive nature of an immunoglobulin-superfamily receptor by usurping the ligand-binding site of this molecule to attach to the cell surface. The dissociation constant (K(D)) of the interaction between sigma1 and JAM-A is 1,000-fold lower than that of the homophilic interaction between JAM-A molecules, indicating that JAM-A strongly prefers sigma1 as a ligand. Analysis of reovirus mutants engineered by plasmid-based reverse genetics revealed residues in sigma1 required for binding to JAM-A and infectivity of cultured cells. These studies define biophysical mechanisms of reovirus cell attachment and provide a platform for manipulating reovirus tropism to enhance vector targeting.
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===Structure of Reovirus sigma1 in Complex with Its Receptor Junctional Adhesion Molecule-A===
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Structure of reovirus sigma1 in complex with its receptor junctional adhesion molecule-A.,Kirchner E, Guglielmi KM, Strauss HM, Dermody TS, Stehle T PLoS Pathog. 2008 Dec;4(12):e1000235. Epub 2008 Dec 12. PMID:19079583<ref>PMID:19079583</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3eoy" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_19079583}}, adds the Publication Abstract to the page
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*[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 19079583 is the PubMed ID number.
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_19079583}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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3EOY is a 12 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Reovirus_sp. Reovirus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EOY OCA].
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==Reference==
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<ref group="xtra">PMID:19079583</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Reovirus sp.]]
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[[Category: Large Structures]]
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[[Category: Dermody, T S.]]
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[[Category: Mammalian orthoreovirus 3 Dearing]]
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[[Category: Guglielmi, K M.]]
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[[Category: Dermody TS]]
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[[Category: Kirchner, E.]]
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[[Category: Guglielmi KM]]
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[[Category: Stehle, T.]]
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[[Category: Kirchner E]]
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[[Category: Beta-barrel]]
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[[Category: Stehle T]]
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[[Category: Beta-spiral repeat]]
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[[Category: Greek key motif]]
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[[Category: Immunoglobulin superfamily]]
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[[Category: Protein complex]]
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[[Category: Trimer]]
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[[Category: Viral protein/cell adhesion complex]]
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[[Category: Virus receptor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 11:39:05 2010''
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Current revision

Structure of Reovirus sigma1 in Complex with Its Receptor Junctional Adhesion Molecule-A

PDB ID 3eoy

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