|
|
| (7 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | {{Seed}} | |
| - | [[Image:2zsi.png|left|200px]] | |
| | | | |
| - | <!--
| + | ==Structural basis of gibberellin(GA4)-induced DELLA recognition by the gibberellin receptor== |
| - | The line below this paragraph, containing "STRUCTURE_2zsi", creates the "Structure Box" on the page.
| + | <StructureSection load='2zsi' size='340' side='right'caption='[[2zsi]], [[Resolution|resolution]] 1.80Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[2zsi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZSI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZSI FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | --> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GA4:GIBBERELLIN+A4'>GA4</scene></td></tr> |
| - | {{STRUCTURE_2zsi| PDB=2zsi | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zsi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zsi OCA], [https://pdbe.org/2zsi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zsi RCSB], [https://www.ebi.ac.uk/pdbsum/2zsi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zsi ProSAT]</span></td></tr> |
| | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/GID1A_ARATH GID1A_ARATH] Functions as soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active gibberellin GA4, but has no affinity for the biologically inactive GAs. In response to GA, interacts with specific DELLA proteins, known as repressors of GA-induced growth, and targets them for degradation via proteasome. Seems to be required for GA signaling that controls root growth, seed germination, stem elongation and flower development. Partially redundant with GID1B and GID1C.<ref>PMID:16709201</ref> <ref>PMID:17194763</ref> <ref>PMID:17521411</ref> |
| | + | <div style="background-color:#fffaf0;"> |
| | + | == Publication Abstract from PubMed == |
| | + | Gibberellins control a range of growth and developmental processes in higher plants and have been widely used in the agricultural industry. By binding to a nuclear receptor, GIBBERELLIN INSENSITIVE DWARF1 (GID1), gibberellins regulate gene expression by promoting degradation of the transcriptional regulator DELLA proteins, including GIBBERELLIN INSENSITIVE (GAI). The precise manner in which GID1 discriminates and becomes activated by bioactive gibberellins for specific binding to DELLA proteins remains unclear. Here we present the crystal structure of a ternary complex of Arabidopsis thaliana GID1A, a bioactive gibberellin and the amino-terminal DELLA domain of GAI. In this complex, GID1A occludes gibberellin in a deep binding pocket covered by its N-terminal helical switch region, which in turn interacts with the DELLA domain containing DELLA, VHYNP and LExLE motifs. Our results establish a structural model of a plant hormone receptor that is distinct from the mechanism of the hormone perception and effector recognition of the known auxin receptors. |
| | | | |
| - | ===Structural basis of gibberellin(GA4)-induced DELLA recognition by the gibberellin receptor===
| + | Gibberellin-induced DELLA recognition by the gibberellin receptor GID1.,Murase K, Hirano Y, Sun TP, Hakoshima T Nature. 2008 Nov 27;456(7221):459-63. PMID:19037309<ref>PMID:19037309</ref> |
| | | | |
| - | | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | <!--
| + | </div> |
| - | The line below this paragraph, {{ABSTRACT_PUBMED_19037309}}, adds the Publication Abstract to the page
| + | <div class="pdbe-citations 2zsi" style="background-color:#fffaf0;"></div> |
| - | (as it appears on PubMed at http://www.pubmed.gov), where 19037309 is the PubMed ID number.
| + | == References == |
| - | -->
| + | <references/> |
| - | {{ABSTRACT_PUBMED_19037309}}
| + | __TOC__ |
| - | | + | </StructureSection> |
| - | ==About this Structure== | + | |
| - | 2ZSI is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZSI OCA].
| + | |
| - | | + | |
| - | ==Reference== | + | |
| - | <ref group="xtra">PMID:19037309</ref><references group="xtra"/> | + | |
| | [[Category: Arabidopsis thaliana]] | | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Hakoshima, T.]] | + | [[Category: Large Structures]] |
| - | [[Category: Hirano, Y.]] | + | [[Category: Hakoshima T]] |
| - | [[Category: Murase, K.]]
| + | [[Category: Hirano Y]] |
| - | [[Category: Sun, T P.]]
| + | [[Category: Murase K]] |
| - | [[Category: Alpha/beta-hydrolase fold]] | + | [[Category: Sun TP]] |
| - | [[Category: Carboxyesterase family]] | + | |
| - | [[Category: Developmental protein]] | + | |
| - | [[Category: Gibberellin signaling pathway]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Nucleus]]
| + | |
| - | [[Category: Phosphoprotein]]
| + | |
| - | [[Category: Plant hormone receptor]]
| + | |
| - | [[Category: Receptor]]
| + | |
| - | [[Category: Repressor]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| - | [[Category: Ubl conjugation]]
| + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 11:44:24 2010''
| + | |
| Structural highlights
Function
GID1A_ARATH Functions as soluble gibberellin (GA) receptor. GA is an essential hormone that regulates growth and development in plants. Binds with high affinity the biologically active gibberellin GA4, but has no affinity for the biologically inactive GAs. In response to GA, interacts with specific DELLA proteins, known as repressors of GA-induced growth, and targets them for degradation via proteasome. Seems to be required for GA signaling that controls root growth, seed germination, stem elongation and flower development. Partially redundant with GID1B and GID1C.[1] [2] [3]
Publication Abstract from PubMed
Gibberellins control a range of growth and developmental processes in higher plants and have been widely used in the agricultural industry. By binding to a nuclear receptor, GIBBERELLIN INSENSITIVE DWARF1 (GID1), gibberellins regulate gene expression by promoting degradation of the transcriptional regulator DELLA proteins, including GIBBERELLIN INSENSITIVE (GAI). The precise manner in which GID1 discriminates and becomes activated by bioactive gibberellins for specific binding to DELLA proteins remains unclear. Here we present the crystal structure of a ternary complex of Arabidopsis thaliana GID1A, a bioactive gibberellin and the amino-terminal DELLA domain of GAI. In this complex, GID1A occludes gibberellin in a deep binding pocket covered by its N-terminal helical switch region, which in turn interacts with the DELLA domain containing DELLA, VHYNP and LExLE motifs. Our results establish a structural model of a plant hormone receptor that is distinct from the mechanism of the hormone perception and effector recognition of the known auxin receptors.
Gibberellin-induced DELLA recognition by the gibberellin receptor GID1.,Murase K, Hirano Y, Sun TP, Hakoshima T Nature. 2008 Nov 27;456(7221):459-63. PMID:19037309[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nakajima M, Shimada A, Takashi Y, Kim YC, Park SH, Ueguchi-Tanaka M, Suzuki H, Katoh E, Iuchi S, Kobayashi M, Maeda T, Matsuoka M, Yamaguchi I. Identification and characterization of Arabidopsis gibberellin receptors. Plant J. 2006 Jun;46(5):880-9. PMID:16709201 doi:http://dx.doi.org/10.1111/j.1365-313X.2006.02748.x
- ↑ Griffiths J, Murase K, Rieu I, Zentella R, Zhang ZL, Powers SJ, Gong F, Phillips AL, Hedden P, Sun TP, Thomas SG. Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis. Plant Cell. 2006 Dec;18(12):3399-414. Epub 2006 Dec 28. PMID:17194763 doi:http://dx.doi.org/10.1105/tpc.106.047415
- ↑ Iuchi S, Suzuki H, Kim YC, Iuchi A, Kuromori T, Ueguchi-Tanaka M, Asami T, Yamaguchi I, Matsuoka M, Kobayashi M, Nakajima M. Multiple loss-of-function of Arabidopsis gibberellin receptor AtGID1s completely shuts down a gibberellin signal. Plant J. 2007 Jun;50(6):958-66. Epub 2007 May 23. PMID:17521411 doi:http://dx.doi.org/10.1111/j.1365-313X.2007.03098.x
- ↑ Murase K, Hirano Y, Sun TP, Hakoshima T. Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. Nature. 2008 Nov 27;456(7221):459-63. PMID:19037309 doi:http://dx.doi.org/10.1038/nature07519
|
