2vye

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (15:37, 13 December 2023) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2vye.png|left|200px]]
 
-
<!--
+
==Crystal Structure of the DnaC-ssDNA complex==
-
The line below this paragraph, containing "STRUCTURE_2vye", creates the "Structure Box" on the page.
+
<StructureSection load='2vye' size='340' side='right'caption='[[2vye]], [[Resolution|resolution]] 4.10&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2vye]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VYE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VYE FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.1&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vye OCA], [https://pdbe.org/2vye PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vye RCSB], [https://www.ebi.ac.uk/pdbsum/2vye PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vye ProSAT]</span></td></tr>
-
{{STRUCTURE_2vye| PDB=2vye | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q5KU75_GEOKA Q5KU75_GEOKA]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vy/2vye_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vye ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
DNA helicases are motor proteins that play essential roles in DNA replication, repair and recombination. In the replicative hexameric helicase, the fundamental reaction is the unwinding of duplex DNA; however, our understanding of this function remains vague due to insufficient structural information. Here, we report two crystal structures of the DnaB-family replicative helicase from Geobacillus kaustophilus HTA426 (GkDnaC) in the apo-form and bound to single-stranded DNA (ssDNA). The GkDnaC-ssDNA complex structure reveals that three symmetrical basic grooves on the interior surface of the hexamer individually encircle ssDNA. The ssDNA-binding pockets in this structure are directed toward the N-terminal domain collar of the hexameric ring, thus orienting the ssDNA toward the DnaG primase to facilitate the synthesis of short RNA primers. These findings provide insight into the mechanism of ssDNA binding and provide a working model to establish a novel mechanism for DNA translocation at the replication fork.
-
===CRYSTAL STRUCTURE OF THE DNAC-SSDNA COMPLEX===
+
The crystal structure of a replicative hexameric helicase DnaC and its complex with single-stranded DNA.,Lo YH, Tsai KL, Sun YJ, Chen WT, Huang CY, Hsiao CD Nucleic Acids Res. 2008 Dec 15. PMID:19074952<ref>PMID:19074952</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2vye" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_19074952}}, adds the Publication Abstract to the page
+
*[[DnaC helicase loader|DnaC helicase loader]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 19074952 is the PubMed ID number.
+
*[[Helicase 3D structures|Helicase 3D structures]]
-
-->
+
== References ==
-
{{ABSTRACT_PUBMED_19074952}}
+
<references/>
-
 
+
__TOC__
-
==About this Structure==
+
</StructureSection>
-
2VYE is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VYE OCA].
+
[[Category: Escherichia coli]]
-
 
+
[[Category: Geobacillus kaustophilus HTA426]]
-
==Reference==
+
[[Category: Large Structures]]
-
<ref group="xtra">PMID:19074952</ref><references group="xtra"/>
+
[[Category: Hsiao CD]]
-
[[Category: Geobacillus kaustophilus hta426]]
+
[[Category: Lo YH]]
-
[[Category: Hsiao, C D.]]
+
[[Category: Sun YJ]]
-
[[Category: Lo, Y H.]]
+
[[Category: Tsai KL]]
-
[[Category: Sun, Y J.]]
+
-
[[Category: Tsai, K L.]]
+
-
[[Category: Atp-binding]]
+
-
[[Category: Dna replication]]
+
-
[[Category: Dnac]]
+
-
[[Category: Helicase]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Nucleotide-binding]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 11:45:49 2010''
+

Current revision

Crystal Structure of the DnaC-ssDNA complex

PDB ID 2vye

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vye"
Personal tools