3exl

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{{Seed}}
 
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[[Image:3exl.png|left|200px]]
 
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==Crystal Structure of a p53 Core Tetramer Bound to DNA==
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The line below this paragraph, containing "STRUCTURE_3exl", creates the "Structure Box" on the page.
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<StructureSection load='3exl' size='340' side='right'caption='[[3exl]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3exl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EXL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EXL FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3exl| PDB=3exl | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3exl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3exl OCA], [https://pdbe.org/3exl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3exl RCSB], [https://www.ebi.ac.uk/pdbsum/3exl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3exl ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/P53_MOUSE P53_MOUSE] Note=p53 is found in increased amounts in a wide variety of transformed cells. p53 is frequently mutated or inactivated in many types of cancer.
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== Function ==
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[https://www.uniprot.org/uniprot/P53_MOUSE P53_MOUSE] Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; te function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression (By similarity). Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis, but seems to have to effect on cell-cycle regulation.<ref>PMID:19556538</ref> <ref>PMID:20673990</ref> <ref>PMID:22726440</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ex/3exl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3exl ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The tumor suppressor p53 regulates downstream genes in response to many cellular stresses and is frequently mutated in human cancers. Here, we report the use of a crosslinking strategy to trap a tetrameric p53 DNA-binding domain (p53DBD) bound to DNA and the X-ray crystal structure of the protein/DNA complex. The structure reveals that two p53DBD dimers bind to B form DNA with no relative twist and that a p53 tetramer can bind to DNA without introducing significant DNA bending. The numerous dimer-dimer interactions involve several strictly conserved residues, thus suggesting a molecular basis for p53DBD-DNA binding cooperativity. Surface residue conservation of the p53DBD tetramer bound to DNA highlights possible regions of other p53 domain or p53 cofactor interactions.
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===Crystal Structure of a p53 Core Tetramer Bound to DNA===
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Crystal structure of a p53 core tetramer bound to DNA.,Malecka KA, Ho WC, Marmorstein R Oncogene. 2009 Jan 22;28(3):325-33. Epub 2008 Nov 3. PMID:18978813<ref>PMID:18978813</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3exl" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18978813}}, adds the Publication Abstract to the page
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*[[P53 3D structures|P53 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18978813 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18978813}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3EXL is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EXL OCA].
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==Reference==
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<ref group="xtra">PMID:18978813</ref><references group="xtra"/>
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[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Malecka, K A.]]
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[[Category: Malecka KA]]
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[[Category: Acetylation]]
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[[Category: Activator]]
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[[Category: Anti-oncogene]]
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[[Category: Apoptosis]]
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[[Category: Cell cycle]]
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[[Category: Covalent protein-rna linkage]]
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[[Category: Cytoplasm]]
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[[Category: Disease mutation]]
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[[Category: Dna-binding]]
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[[Category: Endoplasmic reticulum]]
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[[Category: Metal-binding]]
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[[Category: Methylation]]
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[[Category: Nucleus]]
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[[Category: Phosphoprotein]]
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[[Category: Protein-dna complex]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transcription/dna complex]]
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[[Category: Ubl conjugation]]
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[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 11:53:52 2010''
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Current revision

Crystal Structure of a p53 Core Tetramer Bound to DNA

PDB ID 3exl

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