2qlx

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{{Seed}}
 
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[[Image:2qlx.png|left|200px]]
 
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==Crystal structure of rhamnose mutarotase RhaU of Rhizobium leguminosarum in complex with L-Rhamnose==
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The line below this paragraph, containing "STRUCTURE_2qlx", creates the "Structure Box" on the page.
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<StructureSection load='2qlx' size='340' side='right'caption='[[2qlx]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qlx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_leguminosarum_bv._trifolii Rhizobium leguminosarum bv. trifolii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QLX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=RM4:6-DEOXY-BETA-L-MANNOPYRANOSE'>RM4</scene></td></tr>
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{{STRUCTURE_2qlx| PDB=2qlx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qlx OCA], [https://pdbe.org/2qlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qlx RCSB], [https://www.ebi.ac.uk/pdbsum/2qlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qlx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RHAM_RHILT RHAM_RHILT] Involved in the anomeric conversion of L-rhamnose.<ref>PMID:18156270</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ql/2qlx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qlx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Of the nine genes comprising the L-rhamnose operon of Rhizobium leguminosarum, rhaU has not been assigned a function. The construction of a Delta rhaU strain revealed a growth phenotype that was slower than that of the wild-type strain, although the ultimate cell yields were equivalent. The transport of L-rhamnose into the cell and the rate of its phosphorylation were unaffected by the mutation. RhaU exhibits weak sequence similarity to the formerly hypothetical protein YiiL of Escherichia coli that has recently been characterized as an L-rhamnose mutarotase. To characterize RhaU further, a His-tagged variant of the protein was prepared and subjected to mass spectrometry analysis, confirming the subunit size and demonstrating its dimeric structure. After crystallization, the structure was refined to a 1.6-A resolution to reveal a dimer in the asymmetric unit with a very similar structure to that of YiiL. Soaking a RhaU crystal with L-rhamnose resulted in the appearance of beta-L-rhamnose in the active site.
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===Crystal structure of rhamnose mutarotase RhaU of Rhizobium leguminosarum in complex with L-Rhamnose===
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RhaU of Rhizobium leguminosarum is a rhamnose mutarotase.,Richardson JS, Carpena X, Switala J, Perez-Luque R, Donald LJ, Loewen PC, Oresnik IJ J Bacteriol. 2008 Apr;190(8):2903-10. Epub 2007 Dec 21. PMID:18156270<ref>PMID:18156270</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18156270}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2qlx" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18156270 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18156270}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2QLX is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Rhizobium_leguminosarum_bv._trifolii Rhizobium leguminosarum bv. trifolii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QLX OCA].
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==Reference==
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<ref group="xtra">PMID:18156270</ref><references group="xtra"/>
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[[Category: Rhizobium leguminosarum bv. trifolii]]
[[Category: Rhizobium leguminosarum bv. trifolii]]
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[[Category: Carpena, X.]]
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[[Category: Carpena X]]
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[[Category: Loewen, P C.]]
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[[Category: Loewen PC]]
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[[Category: Carbohydrate metabolism]]
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[[Category: Cytoplasm]]
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[[Category: Isomerase]]
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[[Category: Mutarotase]]
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[[Category: Plasmid]]
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[[Category: Rhamnose metabolism]]
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[[Category: Rhau]]
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[[Category: Rhizobium leguminosarum. l-rhamnose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 12:01:30 2010''
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Current revision

Crystal structure of rhamnose mutarotase RhaU of Rhizobium leguminosarum in complex with L-Rhamnose

PDB ID 2qlx

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