3nii

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{{Seed}}
 
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[[Image:3nii.png|left|200px]]
 
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==The structure of UBR box (KIAA)==
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The line below this paragraph, containing "STRUCTURE_3nii", creates the "Structure Box" on the page.
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<StructureSection load='3nii' size='340' side='right'caption='[[3nii]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3nii]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NII FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_3nii| PDB=3nii | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nii OCA], [https://pdbe.org/3nii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nii RCSB], [https://www.ebi.ac.uk/pdbsum/3nii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nii ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UBR1_YEAST UBR1_YEAST] Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ni/3nii_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nii ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The N-end rule pathway is a regulated proteolytic system that targets proteins containing destabilizing N-terminal residues (N-degrons) for ubiquitination and proteasomal degradation in eukaryotes. The N-degrons of type 1 substrates contain an N-terminal basic residue that is recognized by the UBR box domain of the E3 ubiquitin ligase UBR1. We describe structures of the UBR box of Saccharomyces cerevisiae UBR1 alone and in complex with N-degron peptides, including that of the cohesin subunit Scc1, which is cleaved and targeted for degradation at the metaphase-anaphase transition. The structures reveal a previously unknown protein fold that is stabilized by a novel binuclear zinc center. N-terminal arginine, lysine or histidine side chains of the N-degron are coordinated in a multispecific binding pocket. Unexpectedly, the structures together with our in vitro biochemical and in vivo pulse-chase analyses reveal a previously unknown modulation of binding specificity by the residue at position 2 of the N-degron.
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===The structure of UBR box (KIAA)===
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Structural basis for the recognition of N-end rule substrates by the UBR box of ubiquitin ligases.,Choi WS, Jeong BC, Joo YJ, Lee MR, Kim J, Eck MJ, Song HK Nat Struct Mol Biol. 2010 Sep 12. PMID:20835240<ref>PMID:20835240</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3nii" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20835240}}, adds the Publication Abstract to the page
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*[[Ubiquitin protein ligase 3D structures|Ubiquitin protein ligase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20835240 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20835240}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3NII is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NII OCA].
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==Reference==
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<ref group="xtra">PMID:20835240</ref><references group="xtra"/>
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Choi, W S.]]
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[[Category: Choi WS]]
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[[Category: Jeong, B C.]]
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[[Category: Jeong B-C]]
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[[Category: Lee, M R.]]
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[[Category: Lee M-R]]
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[[Category: Song, H K.]]
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[[Category: Song HK]]
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[[Category: E3 ubiquitin ligase]]
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[[Category: Ligase]]
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[[Category: Metal binding protein]]
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[[Category: N-end rule]]
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[[Category: Ubr box]]
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[[Category: Zinc-binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 14:19:55 2010''
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Current revision

The structure of UBR box (KIAA)

PDB ID 3nii

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