3omy

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'''Unreleased structure'''
 
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The entry 3omy is ON HOLD until Paper Publication
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==Crystal structure of the pED208 TraM N-terminal domain==
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<StructureSection load='3omy' size='340' side='right'caption='[[3omy]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3omy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OMY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3omy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3omy OCA], [https://pdbe.org/3omy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3omy RCSB], [https://www.ebi.ac.uk/pdbsum/3omy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3omy ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRAM8_ECOLX TRAM8_ECOLX] Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site (By similarity). TraM binds to three principal regions in the oriT (transfer origin) region; 2 are required for autoregulation while the other is required for plasmid transfer. Plasmid specificity is conferred by the TraD-TraM pair.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The conjugative transfer of F-like plasmids such as F, R1, R100 and pED208, between bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers bridge the origin of transfer (oriT) to a key component of the conjugative pore, the coupling protein TraD. Here we show that TraM recognizes a high-affinity DNA-binding site, sbmA, as a cooperative dimer of tetramers. The crystal structure of the TraM-sbmA complex from the plasmid pED208 shows that binding cooperativity is mediated by DNA kinking and unwinding, without any direct contact between tetramers. Sequence-specific DNA recognition is carried out by TraM's N-terminal ribbon-helix-helix (RHH) domains, which bind DNA in a staggered arrangement. We demonstrate that both DNA-binding specificity, as well as selective interactions between TraM and the C-terminal tail of its cognate TraD mediate conjugation specificity within the F-like family of plasmids. The ability of TraM to cooperatively bind DNA without interaction between tetramers leaves the C-terminal TraM tetramerization domains free to make multiple interactions with TraD, driving recruitment of the plasmid to the conjugative pore.
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Authors: Wong, J.J.W., Lu, J., Edwards, R.A., Frost, L.S., Mark Glover, J.N.
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Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM.,Wong JJ, Lu J, Edwards RA, Frost LS, Glover JN Nucleic Acids Res. 2011 May 11. PMID:21565799<ref>PMID:21565799</ref>
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Description: Crystal structure of the pED208 TraM N-terminal domain
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 14:33:45 2010''
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<div class="pdbe-citations 3omy" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Edwards RA]]
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[[Category: Frost LS]]
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[[Category: Lu J]]
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[[Category: Mark Glover JN]]
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[[Category: Wong JJW]]

Current revision

Crystal structure of the pED208 TraM N-terminal domain

PDB ID 3omy

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