3n0a

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{{Seed}}
 
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[[Image:3n0a.jpg|left|200px]]
 
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==Crystal structure of auxilin (40-400)==
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The line below this paragraph, containing "STRUCTURE_3n0a", creates the "Structure Box" on the page.
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<StructureSection load='3n0a' size='340' side='right'caption='[[3n0a]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3n0a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N0A FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_3n0a| PDB=3n0a | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n0a OCA], [https://pdbe.org/3n0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n0a RCSB], [https://www.ebi.ac.uk/pdbsum/3n0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n0a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AUXI_BOVIN AUXI_BOVIN] Recruits HSPA8/HSC70 to clathrin-coated vesicles and promotes uncoating of clathrin-coated vesicles.<ref>PMID:15502813</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/3n0a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n0a ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Auxilin, a J-domain containing protein, recruits the Hsc70 uncoating ATPase to newly budded clathrin-coated vesicles. The timing of auxilin arrival determines that uncoating will commence only after the clathrin lattice has fully assembled and after membrane fission is complete. Auxilin has a region resembling PTEN, a PI3P phosphatase. We have determined the crystal structure of this region of bovine auxilin 1; it indeed resembles PTEN closely. A change in the structure of the P loop accounts for the lack of phosphatase activity. Inclusion of phosphatidylinositol phosphates substantially enhances liposome binding by wild-type auxilin, but not by various mutants bearing changes in loops of the C2 domain. Nearly all these mutations also prevent recruitment of auxilin to newly budded coated vesicles. We propose a specific geometry for auxilin association with a membrane bilayer and discuss implications of this model for the mechanism by which auxilin detects separation of a vesicle from its parent membrane.
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===Crystal structure of auxilin (40-400)===
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Structure of the PTEN-like region of auxilin, a detector of clathrin-coated vesicle budding.,Guan R, Han D, Harrison SC, Kirchhausen T Structure. 2010 Sep 8;18(9):1191-8. PMID:20826345<ref>PMID:20826345</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20826345}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3n0a" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20826345 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20826345}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3N0A is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N0A OCA].
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==Reference==
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<ref group="xtra">PMID:20826345</ref><references group="xtra"/>
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Protein-tyrosine-phosphatase]]
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[[Category: Large Structures]]
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[[Category: Dai, H.]]
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[[Category: Dai H]]
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[[Category: Guan, R.]]
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[[Category: Guan R]]
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[[Category: Harrison, S C.]]
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[[Category: Harrison SC]]
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[[Category: Kirchhausen, T.]]
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[[Category: Kirchhausen T]]
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[[Category: C2 domain]]
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[[Category: Hydrolase]]
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[[Category: Phosphatase-like domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 14:46:44 2010''
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Current revision

Crystal structure of auxilin (40-400)

PDB ID 3n0a

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