3nbh

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{{Seed}}
 
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[[Image:3nbh.jpg|left|200px]]
 
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==Crystal structure of human RMI1C-RMI2 complex==
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The line below this paragraph, containing "STRUCTURE_3nbh", creates the "Structure Box" on the page.
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<StructureSection load='3nbh' size='340' side='right'caption='[[3nbh]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3nbh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NBH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3nbh| PDB=3nbh | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nbh OCA], [https://pdbe.org/3nbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nbh RCSB], [https://www.ebi.ac.uk/pdbsum/3nbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nbh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RMI1_HUMAN RMI1_HUMAN] Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.<ref>PMID:15775963</ref> <ref>PMID:16595695</ref> <ref>PMID:16537486</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nb/3nbh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3nbh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mutations in BLM, a RecQ-like helicase, are linked to the autosomal recessive cancer-prone disorder Bloom's syndrome. BLM associates with topoisomerase (Topo) IIIalpha, RMI1, and RMI2 to form the BLM complex that is essential for genome stability. The RMI1-RMI2 heterodimer stimulates the dissolution of double Holliday junction into non-crossover recombinants mediated by BLM-Topo IIIalpha and is essential for stabilizing the BLM complex. However, the molecular basis of these functions of RMI1 and RMI2 remains unclear. Here we report the crystal structures of multiple domains of RMI1-RMI2, providing direct confirmation of the existence of three oligonucleotide/oligosaccharide binding (OB)-folds in RMI1-RMI2. Our structural and biochemical analyses revealed an unexpected insertion motif in RMI1N-OB, which is important for stimulating the dHJ dissolution. We also revealed the structural basis of the interaction between RMI1C-OB and RMI2-OB and demonstrated the functional importance of the RMI1-RMI2 interaction in genome stability maintenance.
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===Crystal structure of human RMI1C-RMI2 complex===
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Crystal structures of RMI1 and RMI2, two OB-fold regulatory subunits of the BLM complex.,Wang F, Yang Y, Singh TR, Busygina V, Guo R, Wan K, Wang W, Sung P, Meetei AR, Lei M Structure. 2010 Sep 8;18(9):1159-70. PMID:20826342<ref>PMID:20826342</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20826342}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3nbh" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20826342 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20826342}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3NBH is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NBH OCA].
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==Reference==
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<ref group="xtra">PMID:20826342</ref><references group="xtra"/>
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Busygina, V.]]
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[[Category: Large Structures]]
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[[Category: Guo, R.]]
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[[Category: Busygina V]]
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[[Category: Lei, M.]]
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[[Category: Guo R]]
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[[Category: Meetei, A R.]]
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[[Category: Lei M]]
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[[Category: Singh, T R.]]
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[[Category: Meetei AR]]
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[[Category: Sung, P.]]
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[[Category: Singh TR]]
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[[Category: Wan, K.]]
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[[Category: Sung P]]
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[[Category: Wang, F.]]
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[[Category: Wan K]]
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[[Category: Wang, W.]]
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[[Category: Wang F]]
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[[Category: Yang, Y.]]
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[[Category: Wang W]]
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[[Category: Protein binding]]
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[[Category: Yang Y]]
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[[Category: Rpa-like complex]]
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[[Category: Two ob-folds containing complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 14:47:08 2010''
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Current revision

Crystal structure of human RMI1C-RMI2 complex

PDB ID 3nbh

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OCA

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