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3oon

From Proteopedia

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{{Seed}}
 
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[[Image:3oon.jpg|left|200px]]
 
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==The structure of an outer membrance protein from Borrelia burgdorferi B31==
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The line below this paragraph, containing "STRUCTURE_3oon", creates the "Structure Box" on the page.
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<StructureSection load='3oon' size='340' side='right'caption='[[3oon]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3oon]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borbu Borbu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OON OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OON FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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-->
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_3oon| PDB=3oon | SCENE= }}
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BB_0167 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224326 BORBU])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oon OCA], [http://pdbe.org/3oon PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3oon RCSB], [http://www.ebi.ac.uk/pdbsum/3oon PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3oon ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oo/3oon_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3oon ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Salmonella enterica serovar Typhimurium can induce both humoral and cell-mediated responses when establishing itself in the host. These responses are primarily stimulated against the lipopolysaccharide and major outer membrane (OM) proteins. OmpA is one of these major OM proteins. It comprises a N-terminal eight-stranded beta-barrel transmembrane domain and a C-terminal domain (OmpA(CTD) ). The OmpA(CTD) and its homologs are believed to bind to peptidoglycan (PG) within the periplasm, maintaining bacterial osmotic homeostasis and modulating the permeability and integrity of the OM. Here we present the first crystal structures of the OmpA(CTD) from two pathogens: S. typhimurium (STOmpA(CTD) ) in open and closed forms and causative agent of Lyme Disease Borrelia burgdorferi (BbOmpA(CTD) ), in closed form. In the open form of STOmpA(CTD) , an aspartate residue from a long beta2-alpha3 loop points into the binding pocket, suggesting that an anion group such as a carboxylate group from PG is favored at the binding site. In the closed form of STOmpA(CTD) and in the structure of BbOmpA(CTD) , a sulfate group from the crystallization buffer is tightly bound at the binding site. The differences between the closed and open forms of STOmpA(CTD) , suggest a large conformational change that includes an extension of alpha3 helix by ordering a part of beta2-alpha3 loop. We propose that the sulfate anion observed in these structures mimics the carboxylate group of PG when bound to STOmpA(CTD) suggesting PG-anchoring mechanism. In addition, the binding of PG or a ligand mimic may enhance dimerization of STOmpA(CTD) , or possibly that of full length STOmpA.
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===The structure of an outer membrance protein from Borrelia burgdorferi B31===
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Insights into PG-binding, conformational change, and dimerization of the OmpA C-terminal domains from Salmonella enterica serovar Typhimurium and Borrelia burgdorferi.,Tan K, Deatherage Kaiser BL, Wu R, Cuff M, Fan Y, Bigelow L, Jedrzejczak RP, Adkins JN, Cort JR, Babnigg G, Joachimiak A Protein Sci. 2017 Sep;26(9):1738-1748. doi: 10.1002/pro.3209. Epub 2017 Jun 19. PMID:28580643<ref>PMID:28580643</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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3OON is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OON OCA].
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<div class="pdbe-citations 3oon" style="background-color:#fffaf0;"></div>
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[[Category: Borrelia burgdorferi]]
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== References ==
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[[Category: Bigelow, L.]]
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<references/>
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[[Category: Fan, Y.]]
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__TOC__
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[[Category: Feldman, B.]]
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</StructureSection>
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[[Category: Joachimiak, A.]]
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[[Category: Borbu]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Large Structures]]
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[[Category: Bigelow, L]]
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[[Category: Fan, Y]]
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[[Category: Feldman, B]]
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[[Category: Joachimiak, A]]
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[[Category: Structural genomic]]
[[Category: Mcsg]]
[[Category: Mcsg]]
[[Category: Membrane protein]]
[[Category: Membrane protein]]
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[[Category: Midwest center for structural genomic]]
 
[[Category: Outer membrane protein]]
[[Category: Outer membrane protein]]
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[[Category: Protein structure initiative]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Psi-2]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 22 14:50:06 2010''
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Current revision

The structure of an outer membrance protein from Borrelia burgdorferi B31

PDB ID 3oon

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