This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2oa1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2oa1" size="350" color="white" frame="true" align="right" spinBox="true" caption="2oa1, resolution 2.150&Aring;" /> '''Crystal Structure o...)
Current revision (00:16, 28 December 2023) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2oa1.gif|left|200px]]<br /><applet load="2oa1" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2oa1, resolution 2.150&Aring;" />
 
-
'''Crystal Structure of RebH, a FAD-dependent halogenase from Lechevalieria aerocolonigenes, the L-Tryptophan with FAD complex'''<br />
 
-
==About this Structure==
+
==Crystal Structure of RebH, a FAD-dependent halogenase from Lechevalieria aerocolonigenes, the L-Tryptophan with FAD complex==
-
2OA1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=TRP:'>TRP</scene>, <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=ADN:'>ADN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Cl Binding Site For Residue A 2001'>AC1</scene>, <scene name='pdbsite=AC2:TRP Binding Site For Residue A 2002'>AC2</scene>, <scene name='pdbsite=AC3:TRP Binding Site For Residue A 2003'>AC3</scene>, <scene name='pdbsite=AC4:Fad Binding Site For Residue A 2004'>AC4</scene> and <scene name='pdbsite=AC5:Adn Binding Site For Residue B 2005'>AC5</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OA1 OCA].
+
<StructureSection load='2oa1' size='340' side='right'caption='[[2oa1]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==Reference==
+
<table><tr><td colspan='2'>[[2oa1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OA1 FirstGlance]. <br>
-
The structure of flavin-dependent tryptophan 7-halogenase RebH., Bitto E, Huang Y, Bingman CA, Singh S, Thorson JS, Phillips GN Jr, Proteins. 2008 Jan 1;70(1):289-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17876823 17876823]
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
-
[[Category: Lechevalieria aerocolonigenes]]
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=TRP:TRYPTOPHAN'>TRP</scene></td></tr>
-
[[Category: Single protein]]
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oa1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oa1 OCA], [https://pdbe.org/2oa1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oa1 RCSB], [https://www.ebi.ac.uk/pdbsum/2oa1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oa1 ProSAT]</span></td></tr>
-
[[Category: Bingman, C.A.]]
+
</table>
-
[[Category: Bitto, E.]]
+
== Function ==
-
[[Category: Jr., G.N.Phillips.]]
+
[https://www.uniprot.org/uniprot/REBH_LENAE REBH_LENAE] Involved in the biosynthesis of the indolocarbazole antitumor agent rebeccamycin. Catalyzes the chlorination of tryptophan (Trp) at C7 position to yield 7-chlorotryptophan. The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.<ref>PMID:11983340</ref> <ref>PMID:15743914</ref> <ref>PMID:17260957</ref>
-
[[Category: Singh, S.]]
+
== Evolutionary Conservation ==
-
[[Category: ADN]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: CL]]
+
Check<jmol>
-
[[Category: FAD]]
+
<jmolCheckbox>
-
[[Category: TRP]]
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/2oa1_consurf.spt"</scriptWhenChecked>
-
[[Category: biosynthetic protein]]
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
[[Category: flavin]]
+
<text>to colour the structure by Evolutionary Conservation</text>
-
[[Category: flavoprotein]]
+
</jmolCheckbox>
-
[[Category: halogenase]]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oa1 ConSurf].
-
[[Category: rebeccamycin]]
+
<div style="clear:both"></div>
-
[[Category: rebh]]
+
== References ==
-
 
+
<references/>
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 10:41:43 2008''
+
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Lentzea aerocolonigenes]]
 +
[[Category: Bingman CA]]
 +
[[Category: Bitto E]]
 +
[[Category: Phillips Jr GN]]
 +
[[Category: Singh S]]

Current revision

Crystal Structure of RebH, a FAD-dependent halogenase from Lechevalieria aerocolonigenes, the L-Tryptophan with FAD complex

PDB ID 2oa1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2oa1"
Personal tools