2rc3

[[Image:2rc3.jpg|left|200px]]<br /><applet load="2rc3" size="350" color="white" frame="true" align="right" spinBox="true"

caption="2rc3, resolution 1.60&Aring;" />

'''Crystal structure of CBS domain, NE2398'''<br />

==Overview==

We tested the general applicability of in situ proteolysis to form protein, crystals suitable for structure determination by adding a protease, (chymotrypsin or trypsin) digestion step to crystallization trials of 55, bacterial and 14 human proteins that had proven recalcitrant to our best, efforts at crystallization or structure determination. This is a work in, progress; so far we determined structures of 9 bacterial proteins and the, human aminoimidazole ribonucleotide synthetase (AIRS) domain.

 

==About this Structure==

2RC3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea_atcc_19718 Nitrosomonas europaea atcc 19718] with <scene name='pdbligand=BR:'>BR</scene> and <scene name='pdbligand=NAD:'>NAD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RC3 OCA].

 

In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Leo RD, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Nov 4;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17982461 17982461]

[[Category: Nitrosomonas europaea atcc 19718]]

[[Category: Single protein]]

[[Category: Dong, A.]]

[[Category: Edwards, A.M.]]

[[Category: Joachimiak, A.]]

[[Category: Korniyenko, Y.]]

[[Category: MCSG, Midwest.Center.for.Structural.Genomics.]]

[[Category: Savchenko, A.]]

[[Category: Walker, J.R.]]

[[Category: Xu, X.]]

[[Category: Yakunin, A.]]

[[Category: Zheng, H.]]

[[Category: BR]]

[[Category: NAD]]

[[Category: br]]

[[Category: cbs domain]]

[[Category: in situ proteolysis]]

[[Category: mcsg]]

[[Category: midwest center for structural genomics]]

[[Category: protein structure initiative]]

[[Category: psi-2]]

[[Category: structural genomics]]

[[Category: unknown function]]