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1ps1

From Proteopedia

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[[Image:1ps1.gif|left|200px]]<br />
 
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<applet load="1ps1" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ps1, resolution 2.60&Aring;" />
 
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'''PENTALENENE SYNTHASE'''<br />
 
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==Overview==
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==PENTALENENE SYNTHASE==
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The crystal structure of pentalenene synthase at 2.6 angstrom resolution, reveals critical active site features responsible for the cyclization of, farnesyl diphosphate into the tricyclic hydrocarbon pentalenene., Metal-triggered substrate ionization initiates catalysis, and the, alpha-barrel active site serves as a template to channel and stabilize the, conformations of reactive carbocation intermediates through a complex, cyclization cascade. The core active site structure of the enzyme may be, preserved among the greater family of terpenoid synthases, possibly, implying divergence from a common ancestral synthase to satisfy biological, requirements for increasingly diverse natural products.
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<StructureSection load='1ps1' size='340' side='right'caption='[[1ps1]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ps1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_exfoliatus Streptomyces exfoliatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PS1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PS1 FirstGlance]. <br>
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1PS1 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.]] with PBM as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Transferred_entry:_4.2.3.7 Transferred entry: 4.2.3.7]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.5 4.6.1.5]]. Structure known Active Sites: AR1 and AR2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PS1 OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PBM:TRIMETHYL+LEAD+ION'>PBM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ps1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ps1 OCA], [https://pdbe.org/1ps1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ps1 RCSB], [https://www.ebi.ac.uk/pdbsum/1ps1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ps1 ProSAT]</span></td></tr>
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Crystal structure of pentalenene synthase: mechanistic insights on terpenoid cyclization reactions in biology., Lesburg CA, Zhai G, Cane DE, Christianson DW, Science. 1997 Sep 19;277(5333):1820-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9295272 9295272]
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</table>
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[[Category: Single protein]]
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== Function ==
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[[Category: Streptomyces sp.]]
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[https://www.uniprot.org/uniprot/PENA_STREX PENA_STREX] Catalyzes the cyclization of farnesyl diphosphate (FPP) to the tricyclic sesquiterpene pentalenene, which is the hydrocarbon precursor of the pentalenolactone family of antibiotics produced by a variety of Streptomyces species.<ref>PMID:8180213</ref>
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[[Category: Transferred entry: 4.2.3.7]]
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== Evolutionary Conservation ==
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[[Category: Christianson, D.W.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Lesburg, C.A.]]
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Check<jmol>
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[[Category: PBM]]
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<jmolCheckbox>
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[[Category: antibiotic biosynthesis]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/1ps1_consurf.spt"</scriptWhenChecked>
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[[Category: lyase]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: sesquiterpene cyclase]]
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:01:08 2007''
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ps1 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Streptomyces exfoliatus]]
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[[Category: Christianson DW]]
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[[Category: Lesburg CA]]

Current revision

PENTALENENE SYNTHASE

PDB ID 1ps1

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