2xlk

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{{Seed}}
 
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[[Image:2xlk.png|left|200px]]
 
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==Crystal structure of the Csy4-crRNA complex, orthorhombic form==
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The line below this paragraph, containing "STRUCTURE_2xlk", creates the "Structure Box" on the page.
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<StructureSection load='2xlk' size='340' side='right'caption='[[2xlk]], [[Resolution|resolution]] 1.81&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xlk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_UCBPP-PA14 Pseudomonas aeruginosa UCBPP-PA14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XLK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.805&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xlk OCA], [https://pdbe.org/2xlk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xlk RCSB], [https://www.ebi.ac.uk/pdbsum/2xlk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xlk ProSAT]</span></td></tr>
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{{STRUCTURE_2xlk| PDB=2xlk | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAS6_PSEAB CAS6_PSEAB] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units. Absolutely required for crRNA production or stability. Upon expression in E.coli endonucleolytically processes pre-crRNA, although disruption and reconstitution experiments indicate that in situ other genes are also required for processing. Yields 5'-hydroxy and 3'-phosphate groups. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:20829488</ref> <ref>PMID:21398535</ref> <ref>PMID:22522703</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xl/2xlk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xlk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many bacteria and archaea contain clustered regularly interspaced short palindromic repeats (CRISPRs) that confer resistance to invasive genetic elements. Central to this immune system is the production of CRISPR-derived RNAs (crRNAs) after transcription of the CRISPR locus. Here, we identify the endoribonuclease (Csy4) responsible for CRISPR transcript (pre-crRNA) processing in Pseudomonas aeruginosa. A 1.8 angstrom crystal structure of Csy4 bound to its cognate RNA reveals that Csy4 makes sequence-specific interactions in the major groove of the crRNA repeat stem-loop. Together with electrostatic contacts to the phosphate backbone, these enable Csy4 to bind selectively and cleave pre-crRNAs using phylogenetically conserved serine and histidine residues in the active site. The RNA recognition mechanism identified here explains sequence- and structure-specific processing by a large family of CRISPR-specific endoribonucleases.
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===CRYSTAL STRUCTURE OF THE CSY4-CRRNA COMPLEX, ORTHORHOMBIC FORM===
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Sequence- and structure-specific RNA processing by a CRISPR endonuclease.,Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA Science. 2010 Sep 10;329(5997):1355-8. PMID:20829488<ref>PMID:20829488</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xlk" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20829488}}, adds the Publication Abstract to the page
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*[[CRISPR subtype I-F|CRISPR subtype I-F]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20829488 is the PubMed ID number.
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_20829488}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2XLK is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XLK OCA].
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[[Category: Large Structures]]
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[[Category: Pseudomonas aeruginosa UCBPP-PA14]]
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==Reference==
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[[Category: Doudna JA]]
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<ref group="xtra">PMID:20829488</ref><references group="xtra"/>
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[[Category: Haurwitz RE]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Jinek M]]
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[[Category: Doudna, J A.]]
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[[Category: Wiedenheft B]]
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[[Category: Haurwitz, R E.]]
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[[Category: Zhou K]]
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[[Category: Jinek, M.]]
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[[Category: Wiedenheft, B.]]
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[[Category: Zhou, K.]]
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[[Category: Crispr]]
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[[Category: Endoribonuclease]]
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[[Category: Hydrolase-rna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 29 07:40:25 2010''
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Current revision

Crystal structure of the Csy4-crRNA complex, orthorhombic form

PDB ID 2xlk

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