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2x8k

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{{Seed}}
 
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[[Image:2x8k.png|left|200px]]
 
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==Crystal Structure of SPP1 Dit (gp 19.1) Protein, a Paradigm of Hub Adsorption Apparatus in Gram-positive Infecting Phages.==
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The line below this paragraph, containing "STRUCTURE_2x8k", creates the "Structure Box" on the page.
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<StructureSection load='2x8k' size='340' side='right'caption='[[2x8k]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x8k]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_SPP1 Bacillus phage SPP1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X8K FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x8k OCA], [https://pdbe.org/2x8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x8k RCSB], [https://www.ebi.ac.uk/pdbsum/2x8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x8k ProSAT]</span></td></tr>
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{{STRUCTURE_2x8k| PDB=2x8k | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DIT_BPSPP DIT_BPSPP] Forms a 40 Angstroms wide channel at the distal tip of the tail. Remains associated to the tail after DNA ejection.<ref>PMID:20843802</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/2x8k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x8k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Siphophage SPP1 infects the Gram+ bacterium Bacillus subtilis using its long non-contractile tail and tail-tip. Electron microscopy (EM) previously allowed a low-resolution assignment of most orf products belonging to these regions. We report here the structure of the SPP1 distal tail protein (Dit, gp19.1). The combination of X-ray crystallography, EM and light scattering established that Dit is a back-to-back dimer of hexamers. However, Dit fitting in the virion EM maps was only possible with a hexamer located between the tail-tube and the tail-tip. Structure comparison revealed high similarity between Dit and a central component of lactophage baseplates. Sequence similarity search expanded its relatedness to several phage proteins, suggesting that Dit is a docking platform for the tail adsorption apparatus in Siphoviridae infecting Gram+ bacteria and that its architecture is a paradigm for these hub proteins. Dit structural similarity extends also to non-contractile and contractile phage tail proteins (gpVN and XkdM) as well as to components of the bacterial type 6 secretion system, supporting an evolutionary connection between all these devices.
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===CRYSTAL STRUCTURE OF SPP1 DIT (GP 19.1) PROTEIN, A PARADIGM OF HUB ADSORPTION APPARATUS IN GRAM-POSITIVE INFECTING PHAGES.===
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Crystal structure of bacteriophage SPP1 distal tail protein (GP 19.1): a baseplate hub paradigm in gram+ infecting phages.,Veesler D, Robin G, Lichiere J, Auzat I, Tavares P, Bron P, Campanacci V, Cambillau C J Biol Chem. 2010 Sep 15. PMID:20843802<ref>PMID:20843802</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<!--
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20843802}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x8k" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20843802 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20843802}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus phage SPP1]]
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2X8K is a 3 chains structure with sequences from [http://en.wikipedia.org/wiki/Bacillus_phage_spp1 Bacillus phage spp1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8K OCA].
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[[Category: Large Structures]]
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[[Category: Auzat I]]
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==Reference==
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[[Category: Bron P]]
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<ref group="xtra">PMID:20843802</ref><references group="xtra"/>
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[[Category: Cambillau C]]
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[[Category: Bacillus phage spp1]]
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[[Category: Campanacci V]]
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[[Category: Auzat, I.]]
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[[Category: Lichiere J]]
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[[Category: Bron, P.]]
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[[Category: Robin G]]
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[[Category: Cambillau, C.]]
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[[Category: Tavares P]]
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[[Category: Campanacci, V.]]
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[[Category: Veesler D]]
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[[Category: Lichiere, J.]]
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[[Category: Robin, G.]]
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[[Category: Tavares, P.]]
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[[Category: Veesler, D.]]
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[[Category: Distal tail protein]]
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[[Category: Viral protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 29 07:46:13 2010''
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Current revision

Crystal Structure of SPP1 Dit (gp 19.1) Protein, a Paradigm of Hub Adsorption Apparatus in Gram-positive Infecting Phages.

PDB ID 2x8k

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