This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2xj7

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:32, 20 December 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2xj7.jpg|left|200px]]
 
-
<!--
+
==BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine==
-
The line below this paragraph, containing "STRUCTURE_2xj7", creates the "Structure Box" on the page.
+
<StructureSection load='2xj7' size='340' side='right'caption='[[2xj7]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2xj7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron_VPI-5482 Bacteroides thetaiotaomicron VPI-5482]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XJ7 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GC2:6-ACETAMIDO-6-DEOXY-CASTANOSPERMINE'>GC2</scene></td></tr>
-
{{STRUCTURE_2xj7| PDB=2xj7 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xj7 OCA], [https://pdbe.org/2xj7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xj7 RCSB], [https://www.ebi.ac.uk/pdbsum/2xj7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xj7 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/OGA_BACTN OGA_BACTN] Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xj/2xj7_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2xj7 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
To probe increased O-GlcNAc levels as an independent mechanism governing insulin resistance in 3T3-L1 adipocytes, a new class of O-GlcNAcase (OGA) inhibitor was studied. 6-Acetamido-6-deoxy-castanospermine (6-Ac-Cas) is a potent inhibitor of OGA. The structure of 6-Ac-Cas bound in the active site of an OGA homolog reveals structural features contributing to its potency. Treatment of 3T3-L1 adipocytes with 6-Ac-Cas increases O-GlcNAc levels in a dose-dependent manner. These increases in O-GlcNAc levels do not induce insulin resistance functionally, measured using a 2-deoxyglucose (2-DOG) uptake assay, or at the molecular level, determined by evaluating levels of phosphorylated IRS-1 and Akt. These results, and others described, provide a structural blueprint for improved inhibitors and collectively suggest that increased O-GlcNAc levels, brought about by inhibition of OGA, does not by itself cause insulin resistance in 3T3-L1 adipocytes.
-
===BTGH84 IN COMPLEX WITH 6-ACETAMIDO-6-DEOXY-CASTANOSPERMINE===
+
Inhibition of O-GlcNAcase using a potent and cell-permeable inhibitor does not induce insulin resistance in 3T3-L1 adipocytes.,Macauley MS, He Y, Gloster TM, Stubbs KA, Davies GJ, Vocadlo DJ Chem Biol. 2010 Sep 24;17(9):937-48. PMID:20851343<ref>PMID:20851343</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2xj7" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_20851343}}, adds the Publication Abstract to the page
+
*[[Beta-Hexosaminidase|Beta-Hexosaminidase]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 20851343 is the PubMed ID number.
+
*[[Beta-Hexosaminidase 3D structures|Beta-Hexosaminidase 3D structures]]
-
-->
+
*[[O-GlcNAcase|O-GlcNAcase]]
-
{{ABSTRACT_PUBMED_20851343}}
+
== References ==
-
 
+
<references/>
-
==About this Structure==
+
__TOC__
-
2XJ7 is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XJ7 OCA].
+
</StructureSection>
-
 
+
[[Category: Bacteroides thetaiotaomicron VPI-5482]]
-
==Reference==
+
[[Category: Large Structures]]
-
<ref group="xtra">PMID:20851343</ref><references group="xtra"/>
+
[[Category: Davies GJ]]
-
[[Category: Bacteria]]
+
[[Category: He Y]]
-
[[Category: Beta-N-acetylhexosaminidase]]
+
-
[[Category: Davies, G J.]]
+
-
[[Category: He, Y.]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Inhibitor]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 29 08:10:47 2010''
+

Current revision

BtGH84 in complex with 6-acetamido-6-deoxy-castanospermine

PDB ID 2xj7

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xj7"
Personal tools