2qrn

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(New page: 200px<br /><applet load="2qrn" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qrn, resolution 3.40&Aring;" /> '''Human Deoxycytidine ...)
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[[Image:2qrn.jpg|left|200px]]<br /><applet load="2qrn" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qrn, resolution 3.40&Aring;" />
 
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'''Human Deoxycytidine kinase dCMP, UDP, Mg ion product complex'''<br />
 
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==Overview==
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==Human Deoxycytidine kinase dCMP, UDP, Mg ion product complex==
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Human deoxycytidine kinase (dCK) is involved in the, nucleotide-biosynthesis salvage pathway and has also been shown to, phosphorylate several antitumor and antiviral prodrugs. The structures of, dCK alone and the dead-end complex of dCK with substrate nucleoside and, product ADP or UDP have previously been reported; however, there is, currently no structure available for a substrate or product complex. Here, the structures of dCK complexes with the products dCMP, UDP and Mg(2+), ion, and with dAMP, UDP and Mg(2+) ion are reported. Structural, comparisons show that the product complexes with UDP and a dead-end, complex with substrate and UDP have similar active-site conformations.
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<StructureSection load='2qrn' size='340' side='right'caption='[[2qrn]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2qrn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QRN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DCM:2-DEOXYCYTIDINE-5-MONOPHOSPHATE'>DCM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qrn OCA], [https://pdbe.org/2qrn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qrn RCSB], [https://www.ebi.ac.uk/pdbsum/2qrn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qrn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DCK_HUMAN DCK_HUMAN] Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.<ref>PMID:18377927</ref> <ref>PMID:20614893</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/2qrn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qrn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human deoxycytidine kinase (dCK) is involved in the nucleotide-biosynthesis salvage pathway and has also been shown to phosphorylate several antitumor and antiviral prodrugs. The structures of dCK alone and the dead-end complex of dCK with substrate nucleoside and product ADP or UDP have previously been reported; however, there is currently no structure available for a substrate or product complex. Here, the structures of dCK complexes with the products dCMP, UDP and Mg2+ ion, and with dAMP, UDP and Mg2+ ion are reported. Structural comparisons show that the product complexes with UDP and a dead-end complex with substrate and UDP have similar active-site conformations.
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==About this Structure==
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Structures of human deoxycytidine kinase product complexes.,Soriano EV, Clark VC, Ealick SE Acta Crystallogr D Biol Crystallogr. 2007 Dec;63(Pt 12):1201-7. Epub 2007, Nov 16. PMID:18084067<ref>PMID:18084067</ref>
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2QRN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=UDP:'>UDP</scene> and <scene name='pdbligand=DCM:'>DCM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Deoxycytidine_kinase Deoxycytidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.74 2.7.1.74] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QRN OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structures of human deoxycytidine kinase product complexes., Soriano EV, Clark VC, Ealick SE, Acta Crystallogr D Biol Crystallogr. 2007 Dec;63(Pt 12):1201-7. Epub 2007, Nov 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18084067 18084067]
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</div>
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[[Category: Deoxycytidine kinase]]
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<div class="pdbe-citations 2qrn" style="background-color:#fffaf0;"></div>
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[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Ealick, S.E.]]
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[[Category: Soriano, E.V.]]
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[[Category: DCM]]
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[[Category: MG]]
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[[Category: UDP]]
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[[Category: atp-binding]]
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[[Category: deoxycytidine kinase]]
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[[Category: deoxycytidine monophosphate]]
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[[Category: nucleotide-binding]]
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[[Category: nucleus]]
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[[Category: phosphorylation]]
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[[Category: transferase]]
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[[Category: uridine diphosphate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:03:32 2008''
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==See Also==
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*[[Deoxycytidine kinase 3D structures|Deoxycytidine kinase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Ealick SE]]
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[[Category: Soriano EV]]

Current revision

Human Deoxycytidine kinase dCMP, UDP, Mg ion product complex

PDB ID 2qrn

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