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3lky

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{{Seed}}
 
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[[Image:3lky.jpg|left|200px]]
 
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==Monomeric Griffithsin with a Single Gly-Ser Insertion==
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The line below this paragraph, containing "STRUCTURE_3lky", creates the "Structure Box" on the page.
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<StructureSection load='3lky' size='340' side='right'caption='[[3lky]], [[Resolution|resolution]] 1.11&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3lky]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Griffithsia_sp._Q66D336 Griffithsia sp. Q66D336]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LKY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.11&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3lky| PDB=3lky | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lky OCA], [https://pdbe.org/3lky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lky RCSB], [https://www.ebi.ac.uk/pdbsum/3lky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lky ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GRFIN_GRISQ GRFIN_GRISQ]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/3lky_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lky ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mutations were introduced to the domain-swapped homodimer of the antiviral lectin griffithsin (GRFT). Whereas several single and double mutants remained dimeric, insertion of either two or four amino acids at the dimerization interface resulted in a monomeric form of the protein (mGRFT). Monomeric character of the modified proteins was confirmed by sedimentation equilibrium ultracentrifugation and by their high resolution X-ray crystal structures, whereas their binding to carbohydrates was assessed by isothermal titration calorimetry. Cell-based antiviral activity assays utilizing different variants of mGRFT indicated that the monomeric form of the lectin had greatly reduced activity against HIV-1, suggesting that the antiviral activity of GRFT stems from crosslinking and aggregation of viral particles via multivalent interactions between GRFT and oligosaccharides present on HIV envelope glycoproteins. Atomic resolution crystal structure of a complex between mGRFT and nonamannoside revealed that a single mGRFT molecule binds to two different nonamannoside molecules through all three carbohydrate-binding sites present on the monomer.
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===Monomeric Griffithsin with a Single Gly-Ser Insertion===
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Monomerization of viral entry inhibitor griffithsin elucidates the relationship between multivalent binding to carbohydrates and anti-HIV activity.,Moulaei T, Shenoy SR, Giomarelli B, Thomas C, McMahon JB, Dauter Z, O'Keefe BR, Wlodawer A Structure. 2010 Sep 8;18(9):1104-15. PMID:20826337<ref>PMID:20826337</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20826337}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3lky" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20826337 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20826337}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Griffithsia sp. Q66D336]]
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3LKY is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Griffithsia Griffithsia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKY OCA].
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[[Category: Large Structures]]
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[[Category: Moulaei T]]
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==Reference==
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[[Category: Wlodawer A]]
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<ref group="xtra">PMID:20826337</ref><references group="xtra"/>
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[[Category: Griffithsia]]
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[[Category: Moulaei, T.]]
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[[Category: Wlodawer, A.]]
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[[Category: Anti-hiv]]
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[[Category: Gp120]]
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[[Category: Gp41]]
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[[Category: High mannose]]
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[[Category: Jacalin-related]]
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[[Category: Lectin]]
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[[Category: Man9]]
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[[Category: Mannose-binding]]
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[[Category: Sugar binding protein]]
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[[Category: Sugar-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 06:05:27 2010''
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Current revision

Monomeric Griffithsin with a Single Gly-Ser Insertion

PDB ID 3lky

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