3o0t

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{{Seed}}
 
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[[Image:3o0t.jpg|left|200px]]
 
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==Crystal structure of human phosphoglycerate mutase family member 5 (PGAM5) in complex with phosphate==
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The line below this paragraph, containing "STRUCTURE_3o0t", creates the "Structure Box" on the page.
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<StructureSection load='3o0t' size='340' side='right'caption='[[3o0t]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3o0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O0T FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_3o0t| PDB=3o0t | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o0t OCA], [https://pdbe.org/3o0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o0t RCSB], [https://www.ebi.ac.uk/pdbsum/3o0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o0t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PGAM5_HUMAN PGAM5_HUMAN] Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2-dependent gene expression. Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore.<ref>PMID:18387606</ref> <ref>PMID:19590015</ref> <ref>PMID:22265414</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o0/3o0t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3o0t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PGAM5 is a mitochondrial membrane protein that functions as an atypical Ser/Thr phosphatase and is a regulator of oxidative stress response, necroptosis, and autophagy. Here we present several crystal structures of PGAM5 including the activating N-terminal regulatory sequences, providing a model for structural plasticity, dimerization of the catalytic domain, and the assembly into an enzymatically active dodecameric form. Oligomeric states observed in structures were supported by hydrogen exchange mass spectrometry, size-exclusion chromatography, and analytical ultracentrifugation experiments in solution. We report that the catalytically important N-terminal WDPNWD motif acts as a structural integrator assembling PGAM5 into a dodecamer, allosterically activating the phosphatase by promoting an ordering of the catalytic loop. Additionally the observed active site plasticity enabled visualization of essential conformational rearrangements of catalytic elements. The comprehensive biophysical characterization offers detailed structural models of this key mitochondrial phosphatase that has been associated with the development of diverse diseases.
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===Crystal structure of human phosphoglycerate mutase family member 5 (PGAM5) in complex with phosphate===
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Structures of PGAM5 Provide Insight into Active Site Plasticity and Multimeric Assembly.,Chaikuad A, Filippakopoulos P, Marcsisin SR, Picaud S, Schroder M, Sekine S, Ichijo H, Engen JR, Takeda K, Knapp S Structure. 2017 Jul 5;25(7):1089-1099.e3. doi: 10.1016/j.str.2017.05.020. Epub, 2017 Jun 22. PMID:28648608<ref>PMID:28648608</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3o0t" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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3O0T is a 2 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O0T OCA].
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*[[Phosphoglycerate mutase 3D structures|Phosphoglycerate mutase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Phosphoprotein phosphatase]]
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[[Category: Large Structures]]
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[[Category: Alfano, I.]]
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[[Category: Alfano I]]
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[[Category: Arrowsmith, C H.]]
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[[Category: Arrowsmith CH]]
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[[Category: Barr, A.]]
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[[Category: Barr A]]
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[[Category: Bountra, C.]]
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[[Category: Bountra C]]
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[[Category: Chaikuad, A.]]
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[[Category: Chaikuad A]]
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[[Category: Delft, F von.]]
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[[Category: Edwards AM]]
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[[Category: Edwards, A M.]]
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[[Category: Filippakopoulos P]]
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[[Category: Filippakopoulos, P.]]
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[[Category: Ichijo H]]
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[[Category: Ichijo, H.]]
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[[Category: Knapp S]]
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[[Category: Knapp, S.]]
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[[Category: Picaud S]]
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[[Category: Picaud, S.]]
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[[Category: Takeda K]]
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[[Category: SGC, Structural Genomics Consortium.]]
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[[Category: Weigelt J]]
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[[Category: Takeda, K.]]
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[[Category: Von Delft F]]
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[[Category: Weigelt, J.]]
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[[Category: Bxlbv68]]
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[[Category: Hydrolase]]
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[[Category: Mgc5352 protein]]
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[[Category: Mitochondrial protein]]
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[[Category: Pgam5]]
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[[Category: Phosphoglycerate mutase family member 5]]
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[[Category: Serine/threonine phosphatase]]
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[[Category: Sgc]]
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[[Category: Structural genomic]]
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[[Category: Structural genomics consortium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 6 06:07:24 2010''
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Current revision

Crystal structure of human phosphoglycerate mutase family member 5 (PGAM5) in complex with phosphate

PDB ID 3o0t

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