2oqc

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(New page: 200px<br /><applet load="2oqc" size="350" color="white" frame="true" align="right" spinBox="true" caption="2oqc, resolution 2.50&Aring;" /> '''Crystal Structure of...)
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[[Image:2oqc.jpg|left|200px]]<br /><applet load="2oqc" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2oqc, resolution 2.50&Aring;" />
 
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'''Crystal Structure of Penicillin V acylase from Bacillus subtilis'''<br />
 
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==Overview==
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==Crystal Structure of Penicillin V acylase from Bacillus subtilis==
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Penicillin acylase proteins are amidohydrolase enzymes that cleave, penicillins at the amide bond connecting the side chain to their, beta-lactam nucleus. An unannotated protein from Bacillus subtilis has, been expressed in Escherichia coli, purified and confirmed to possess, penicillin V acylase activity. The protein was crystallized using the, hanging-drop vapour-diffusion method from a solution containing 4 M sodium, formate in 100 mM Tris-HCl buffer pH 8.2. Diffraction data were collected, under cryogenic conditions to a spacing of 2.5 A. The crystals belonged to, the orthorhombic space group C222(1), with unit-cell parameters a = 111.0, b = 308.0, c = 56.0 A. The estimated Matthews coefficient was 3.23 A3, Da(-1), corresponding to 62% solvent content. The structure has been, solved using molecular-replacement methods with B. sphaericus penicillin V, acylase (PDB code 2pva) as the search model.
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<StructureSection load='2oqc' size='340' side='right'caption='[[2oqc]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2oqc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OQC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2oqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2oqc OCA], [https://pdbe.org/2oqc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2oqc RCSB], [https://www.ebi.ac.uk/pdbsum/2oqc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2oqc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/YXEI_BACSU YXEI_BACSU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oq/2oqc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2oqc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2OQC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Active as [http://en.wikipedia.org/wiki/Penicillin_amidase Penicillin amidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.11 3.5.1.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA].
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*[[Penicillin acylase|Penicillin acylase]]
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__TOC__
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==Reference==
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</StructureSection>
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Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16511127 16511127]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Penicillin amidase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Brannigan JA]]
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[[Category: Brannigan, J.A.]]
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[[Category: Dodson GG]]
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[[Category: Dodson, G.G.]]
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[[Category: Prabhune AA]]
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[[Category: Prabhune, A.A.]]
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[[Category: Pundle AV]]
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[[Category: Pundle, A.V.]]
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[[Category: Rathinaswamy P]]
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[[Category: Rathinaswamy, P.]]
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[[Category: Sivaraman H]]
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[[Category: Sivaraman, H.]]
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[[Category: Suresh CG]]
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[[Category: Suresh, C.G.]]
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[[Category: bacillus subtilis]]
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[[Category: choloylglycine hydrolase]]
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[[Category: conjugated bile acid hydrolase]]
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[[Category: ntn-hydrolase]]
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[[Category: penicillin v acylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:06:14 2008''
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Current revision

Crystal Structure of Penicillin V acylase from Bacillus subtilis

PDB ID 2oqc

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