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2xsc

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[[Image:2xsc.jpg|left|200px]]
 
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==Crystal structure of the cell-binding B oligomer of verotoxin-1 from E. coli==
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The line below this paragraph, containing "STRUCTURE_2xsc", creates the "Structure Box" on the page.
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<StructureSection load='2xsc' size='340' side='right'caption='[[2xsc]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2xsc]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bov 1bov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XSC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.052&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2xsc| PDB=2xsc | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xsc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xsc OCA], [https://pdbe.org/2xsc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xsc RCSB], [https://www.ebi.ac.uk/pdbsum/2xsc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xsc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/STXB_BPH19 STXB_BPH19] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Shiga toxin family, a group of cytotoxins associated with diarrhoeal diseases and the haemolytic uraemic syndrome, includes Shiga toxin from Shigella dysenteriae type 1 and verotoxins produced by enteropathogenic Escherichia coli. The family belongs to the A-B class of bacterial toxins, which includes the cholera toxin family, pertussis and diphtheria toxins. These toxins all have bipartite structures consisting of an enzymatic A subunit associated with a B oligomer which binds to specific cell-surface receptors, but their amino-acid sequences and pathogenic mechanisms differ. We have determined the crystal structure of the B oligomer of verotoxin-1 from E. coli. The structure unexpectedly resembles that of the B oligomer of the cholera toxin-like heat-labile enterotoxin from E. coli, despite the absence of detectable sequence similarity between these two proteins. This result implies a distant evolutionary relationship between the Shiga toxin and cholera toxin families. We suggest that the cell surface receptor-binding site lies in a cleft between adjacent subunits of the B pentamer, providing a potential target for drugs and vaccines to prevent toxin binding and effect.
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===CRYSTAL STRUCTURE OF THE CELL-BINDING B OLIGOMER OF VEROTOXIN-1 FROM E. COLI===
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Crystal structure of the cell-binding B oligomer of verotoxin-1 from E. coli.,Stein PE, Boodhoo A, Tyrrell GJ, Brunton JL, Read RJ Nature. 1992 Feb 20;355(6362):748-50. PMID:1741063<ref>PMID:1741063</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2xsc" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_1741063}}, adds the Publication Abstract to the page
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*[[Shiga toxin|Shiga toxin]]
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(as it appears on PubMed at http://www.pubmed.gov), where 1741063 is the PubMed ID number.
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*[[Shiga toxin 3D structures|Shiga toxin 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_1741063}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2XSC is a 5 chains structure with sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1bov 1bov]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XSC OCA].
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==Reference==
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<ref group="xtra">PMID:1741063</ref><references group="xtra"/>
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Boodhoo, A.]]
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[[Category: Large Structures]]
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[[Category: Brunton, J L.]]
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[[Category: Boodhoo A]]
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[[Category: Bunkoczi, G.]]
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[[Category: Brunton JL]]
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[[Category: Oeffner, R D.]]
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[[Category: Bunkoczi G]]
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[[Category: Read, R J.]]
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[[Category: Oeffner RD]]
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[[Category: Stein, P E.]]
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[[Category: Read RJ]]
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[[Category: Tyrrell, G J.]]
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[[Category: Stein PE]]
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[[Category: Toxin]]
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[[Category: Tyrrell GJ]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Oct 13 10:42:32 2010''
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Current revision

Crystal structure of the cell-binding B oligomer of verotoxin-1 from E. coli

PDB ID 2xsc

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